De-acetylated chitosan was in use for chitosan based material and for collagen based materials, type-I collagen of bovine, porcine and fish were in use.
Since, no reports are available on the interaction of azelaic acid with Chitosan and type -I collagen, in the present study, we attempted to prepare biopolymers using azelaic acid as cross-linker for the natural polymers chitosan and type-I collagen.
Type-I collagen from bovine skin was extracted according to the procedure followed by Mitra et al.
The powder form of chitosan (1%) and type-I collagen (0.
Thermal decomposition analysis of AA, native and crosslinked biopolymers (chitosan, type-I collagen, AACCH, AACC, GACCH and GACC) were carried out under nitrogen flow (40 & 60 ml [min.
22] the three dimensional structure of type-I collagen was generated using gencollagen program .
Polystyrene 96 well culture plates (Tarson, India) were coated with native chitosan, type-I collagen and AA cross-linked chitosan (AACCH) and type-I collagen (AACC) biopolymers.