Three regions, representing mono-, di- and trisaccharides may be distinguished in the chromatographic profile of honey sugars.
Two trisaccharides, panose and raffinose were also found in the honey samples that we studied.
Gas chromatographicmass spectrometric method for the qualitative and quantitative determination of disaccharides and trisaccharides in honey," Journal of Chromatography A, 1059: 143-148.
The analysis of concordance among the three tests showed substantial agreement between PGL-I ELISA and the ML Flow, probably because the two antigensused in the tests are trisaccharides.
For the lateral flow assay, antigen NT-P-BSA was used, which is a natural trisaccharide antigen linked to bovine serum albumin.
The mannitol in the disaccharide 1-[beta]-D-fructofuranosyl-D-mannitol is randomly linked 1-2 or 6-2 to fructose and addition of an extra fructose, also linked by its 2-position to the remaining 1- or 6- positions of mannitol in the disaccharide, gives the trisaccharide
s] (21) and of kidney, liver, and spleen nonsecretory ribonucleases (22) have shown that this type of RNase carries a tryptophan-linked mannosyl residue and asparagine-to-N-acetylglucosamine-linked trisaccharides
Synthesis of the neoglycoconjugates of phenolic glycolipid-related trisaccharides for the serodiagnosis of leprosy.
PGL-I itself or in the form of semisynthetic neoglycoproteins containing the synthetic terminal disaccharide or the whole trisaccharide chemically conjugated to such as bovine or human serum albumin, has found its greatest utility in the serological diagnosis, confirmation and management of lepromatous leprosy.
The term carbohydrate-deficient transferrin, or CDT, refers to all transferrin components that lack two or four of their terminal trisaccharides
and whose charge corresponds to disialo- (and to a lesser degree, mono- and asialo-) transferrin .
It has also led to illuminating studies on the binding of trisaccharides
to monoclonal antibodies, which in turn have led to Lemieux's, hydrated polar gate theory" for specific molecular recognition.
leprae was performed using the ELISA technique previously described (10) with natural trisaccharide
linked to bovine serum albumin via a phenolic ring (NT-P-BSA) as a semi-synthetic analogue of PGL-I.