Tris

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Tris

Abbreviation for tris(hydroxymethyl)aminomethane and tris(hydroxymethyl)methylamine; a buffer; used as a trivial name.
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The aliquot was mixed vigorously with 10% trichloroacetic acid solution and TAE buffer (100 mM Tris-acetate, 2 mM EDTA, pH 7.8) and then cooled on ice for 20 min.
Total 18,535 17,001 19,647 Products of restriction digests were separated in a 1.0% agarose horizontal gel using TAE (40 mM Tris-acetate; 1 mM EDTA) buffer for 13 h at 30 V (2V/cm).
Description : Engineering, procurement and construction (EPC) contract to build a tris-acetate phosphate (TAP) unit in Umm Wl Phosphate Mine Complex.
Whole insect homogenate (1:60, w/v) was prepared in 50 mM Tris-acetate buffer, pH 7.5 containing I mM dithiothreitol (DTT), 0.15 M NaC1 and 3 mM NaNO3 for estimation of cytoplasmic protease activities.
To illustrate the RNase-inhibiting effect of binding buffer TAAN, we added 30 [micro]L of QIAamp-purified HIV-1 RNA (6.8 x [10.sup.6] IU/mL) to (i) 1000 [micro]L of RNase-free water, (ii) 240 [micro]L of plasma + 760 [micro]L of TAAN, (iii) 240 [micro]L of plasma + 760 [micro]L of RNase-free water, and (iv) 240 [micro]L of plasma + 760 [micro]L of 200 mmol/L Tris-acetate buffer (pH 4.0).
Samples were separated by electrophoresis on a 1% Sea Plaque agarose gel containing ethidium bromide and 40 mmol Tris-acetate 1 mmol EDTA pH 8.3 buffer.
Electrophoresis was performed in 1x Tris-acetate-EDTA buffer (40 mmol/L Tris-acetate, 20 mmol/L sodium acetate, 1 mmol/L EDTA, pH 8.0) at 1800 V-h (e.g., 15 h at 120 V) and at a constant temperature of 60[degrees]C.
The genomic fragments were electrophoresed on a 1% SeaKem agarose gel (FMC Bioproducts, Rockland, ME) using Tris-acetate (TAE) buffer (0.04 M TAE, M EDTA [pH 8.0]).
Bead-bound biotinylated strands were retained on the filter and rinsed in washing buffer (10 mmol/L Tris-acetate, pH 7.6) for 7 s.
Stock solutions of 10X Tris-acetate-EDTA (TAE; 400 mmol/L Tris-acetate, 10 mmol/L EDTA) and Tris-borate-EDTA (TBE; 1.0 mol/L Tris, 0.9 mol/L boric acid, 0.01 mol/L EDTA) were purchased from Life Technologies.
The final reaction mixture was 0.6 mL and contained 2.5 kU/L glucose-6-phosphate dehydrogenase, 0.6 kU/L phosphoglucomutase, 1 mmol/L [NADP.sup.+], 1 mmol/L magnesium acetate, 1 [micro] mol/L glucose 1,6-diphosphate, 16 mmol/L sodium phosphate (pH 7), 1 mmol/L AMP, 5 g/L glycogen, 0.15 mmol/L EDTA, and GPb, as indicated, in 20 mmol/L Tris-acetate buffer (pH 7.4), containing 0.03 mol/L Mg[Cl.sub.2], 0.1 mmol/L Mg[Cl.sub.2], 0.1 g/L bovine serum albumin, 0.5 g/L gelatin, 0.1 g/L Na[N.sub.3], and 0.2 mmol/L N-ethylmaleimide (22).