Carter KM, Woodley CM and Brown RS: A review of tricaine
methanesulfonate for anesthesia of fish.
Physiological stress responses, egg survival and sperm mortality for rainbow trout broodstock anaesthetized with clove oil, tricaine
methanesulfonate or carbon dioxide.
Safest level of tricaine
methanesulfonate (MS222) to induce anesthesia in juveniles matrinxa, Brycon cephalus.
Note: If fish will not be kept after this experiment, they can be euthanized with an overdose of Tricaine
which will allow more time for the students to observe and collect any data required).
Anesthetics include tricaine
methanesulfonate (MS-222) and quinaldine.
Fish were euthanized by an overdose of tricaine
methanesulfonate (MS-222), and then heads, gonads, and entire gastrointestinal tracts were removed, bagged separately, and frozen until further analyses.
Tadpoles were euthanatized by immersion in a tricaine
solution at 250mg/L for 10 minutes.
Allison  found a reduction of brook trout (Salvelinus fontinalis) sperm motility to less than 10 s at tricaine
concentrations ranging from 19 to 75 mg/l, but Billard  found no reductions in rainbow trout (Oncorhynchus mykiss) sperm motility when working with tricaine
concentrations of 4-150 mg/l.
Moribund fish were humanely euthanized by an overdose of tricaine
methanesulfonate (MS-222; Argent Chemical Laboratories, Redmond, WA, USA).
methane-sulphonate (MS-222) they recommended an induction dosage at 100-200 mg [L.
Anesthesia and recovery with tricaine
methanesulfonate, eugenol and thiopental sodium in the carp, Cyprinus carpio.
Retained specimens were euthanized by freezing or by relaxing in a solution of tricaine
methane sulfonate (MS-222).