Future steps, would include producing the transport vesicles
synthetically within the cell by externally supplied origami-based PNA or similar cages, and - much more speculatively - ultimately using encoded DNA and RNAs to provide these functions.
Al-Bassam and his colleagues solved this issue by developing a new technique that involves damming up a single pathway, which creates a backlog of transport vesicles
(little bubbles that travel up and down neurons carrying membrane protein cargo) impregnated with the illuminated proteins.
Proper sorting of secretory proteins requires that cargo molecules be segregated from resident proteins as transport vesicles
We are in particular interested in how crowding of proteins with an asymmetric distribution of mass across the membrane affects formation of transport vesicles