For their propagation, 1000 mL Erlenmeyer flasks containing 600 mL of
thioglycolate broth (8 g/L) were used.
In brief, after intraperitoneal injection of
thioglycolate broth, the peritoneal cells were isolated.
injection of 1 mL of 3%
thioglycolate. Animals were killed under C[O.sub.2] atmosphere, and cells were harvested by washing peritoneal cavities with 3 mL of PBS, pH 7.2, containing 10 IU/ mL heparin.
The IL-10 concentration released in PECs culture treated with 3% sodium
thioglycolate of Swiss mice healthy and infected with S.
At the laboratory the samples were transferred to test tubes containing
thioglycolate broth, a highly nutritious medium for culturing a variety of microorganisms, and were incubated in a bacteriological incubator at 37[degrees] C for 24 hours (Oplustil, 2004).
Specimen was transported in 3 separate containers: 1 mL inoculated into a selective Todd-Hewitt enrichment media to culture for GBS in a glass bottle; 1 mL inoculated into
thioglycolate enrichment media to culture for facultative anaerobes in a glass bottle; 3 mL in a sterile plastic container.
WT, SIRT2KO, and SIRT2KI mice (n = 8 each) were injected with sodium
thioglycolate medium intraperitoneally, as per literature [25].
Culture of the aortic valve initially on
thioglycolate broth showed budding yeast.
However, these aseptic cases may not have underwent appropriate culture evaluation, as a recent case series of 2 patients demonstrated an infection with Propionibacterium acnes when cultured anaerobically for 10 days on
thioglycolate broth [43].
Archived bacterial samples were thawed and inoculated into sodium
thioglycolate broth using sterile inoculating loop, incubated overnight at 37[degrees]C, growth detected by observing turbidity.