For their propagation, 1000 mL Erlenmeyer flasks containing 600 mL of thioglycolate
broth (8 g/L) were used.
In brief, after intraperitoneal injection of thioglycolate
broth, the peritoneal cells were isolated.
injection of 1 mL of 3% thioglycolate
. Animals were killed under C[O.sub.2] atmosphere, and cells were harvested by washing peritoneal cavities with 3 mL of PBS, pH 7.2, containing 10 IU/ mL heparin.
The IL-10 concentration released in PECs culture treated with 3% sodium thioglycolate
of Swiss mice healthy and infected with S.
At the laboratory the samples were transferred to test tubes containing thioglycolate
broth, a highly nutritious medium for culturing a variety of microorganisms, and were incubated in a bacteriological incubator at 37[degrees] C for 24 hours (Oplustil, 2004).
Specimen was transported in 3 separate containers: 1 mL inoculated into a selective Todd-Hewitt enrichment media to culture for GBS in a glass bottle; 1 mL inoculated into thioglycolate
enrichment media to culture for facultative anaerobes in a glass bottle; 3 mL in a sterile plastic container.
WT, SIRT2KO, and SIRT2KI mice (n = 8 each) were injected with sodium thioglycolate
medium intraperitoneally, as per literature .
Culture of the aortic valve initially on thioglycolate
broth showed budding yeast.
However, these aseptic cases may not have underwent appropriate culture evaluation, as a recent case series of 2 patients demonstrated an infection with Propionibacterium acnes when cultured anaerobically for 10 days on thioglycolate
Archived bacterial samples were thawed and inoculated into sodium thioglycolate
broth using sterile inoculating loop, incubated overnight at 37[degrees]C, growth detected by observing turbidity.