As can be seen, at pH 5 and at concentration of salt 5 mM,
theoretical plate number decreased and peak asymmetry increased significantly, and it resulted in the peak broadening and decreased peak resolution.
In the first step, columns of different properties were investigated: Hypersil Gold C8, 5 [micro]m, 150 mm x 4.6 mm ID; Zorbax, 5 [micro]m, 150 mm x 4.6 mm ID; Knauer C8, 5 [micro]m, 250 mm x 4.6 mm ID; and Supelco C18, 5 [micro]m, 250 mm x 4.6 mm ID To choose the most appropriate column, the retention factor, peak asymmetry factor, and height equivalent to a
theoretical plate (HETP), as well as the retention time of ivabradine, were taken into consideration (Table 1).
The plate thickness ([t.sub.p]) in the prototype models was then determined for target
theoretical plate shear strength ([V.sup.*.sub.p]) of about 50% of the total theoretical capacity ([V.sup.*.sub.u]).
The [W.sub.avg] term in equation 1 can be defined in terms of time and
theoretical plate number (N), as shown in equation 2.
Andrea Palladio (1508-80) is best known for his majestic, temple-like buildings, crowning villa landscapes, scenic urban palaces, piazzas, churches, theaters for the Vicentine nobility and the Venetian Republic, and his Quattro libri dell'architettura (Venice, 1570), a
theoretical plate book of classical exempla in the new literary-to-visual tradition of Vitruvian, Albertian, and Bramantean humanist architecture and urbanism.
Height equivalent to one
theoretical plate, H: The distance along the column occupied by one
theoretical plate; H = L/N.
In this paper, to obtain a good separation degree, higher
theoretical plate number, and good symmetry of chromatographic peak, different mobile phase conditions were investigated for system adaptability test.
The Mean
theoretical plate count, based on USP tangent calculations (USP-2014) for metronidazole peak was 9574 (Table-1).
Interestingly, this complex relationship is similar in some respects to the efficiency profile of gas and liquid chromatographic separations, where the maximum efficiency is related to the carrier gas velocity at which the
theoretical plate height is minimized (19).
For peak response, ZChrom chromatography manager software version 4.2.was used to calculate the statistical data of different parameters like, retention time (tR), peak area (A) symmetry factor of SFV and
theoretical plates (N) (Table 1).
In practice, to obtain a TBP curve, distillation columns with 15 or more
theoretical plates and high reflux ratios (often 5:1 or higher) are used.
Column efficiency was investigated by performing system suitability test including parameters like retention time (tR), tailing factor (T),
theoretical plates (N) and resolution (Res).