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DNA isolated from both fresh samples and formalin-fixed, paraffin-embedded tissues had an identical migration pattern on the TGGE analysis.
The liver and bone marrow biopsies were too small for simultaneous analysis by Southern blot and TGGE. The serous effusion demonstrated a monoclonal population by TCR[Beta] gene analysis.
Detection of a predominant monoclonal DNA fragment by TGGE relies on the same principle on which DGGE is based.
In our study, the sensitivity of the TGGE was 0.1% when MOLT-16 DNA was serially diluted in DNA from reactive lymphoid tissue.
The different migration pattern of PCR products detected by TGGE is particularly important for routine diagnostic analysis, since PCR contamination may cause false positivity.
Scheller et al used TCR[Gamma] primers for PCR amplification and compared heteroduplex analysis, sequencing gel analysis, and TGGE for detection of amplification products.
Separate biopsies isolated from 2 patients with PTLD (3 in one and 2 in the other) showed similar migration patterns by TGGE in each individual, indicating the same clonal origin.
However, it indicates that the results of TGGE should always be interpreted with clinical and histologic findings.
We therefore strongly recommend the use of screening strategies, such as TGGE, capable of detecting all known functionally relevant sequence variations of the apo B-100 receptor-binding domain.
 Nonstandard abbreviafions: apo B, apolipoprotein B; LDL-C, LDL-cholesterol; FDB, familial defecfive apo B-100; CAD, coronary artery disease; TGGE, temperature gradient gel electrophoresis; HLP, hyperlipoproteinemia; HDL-C, HDL-cholesterol; RFLP, restricfion fragment length polymorphism; and SSCP, single-strand conformation polymorphism.
Positions and sequences of primers used for TGGE PCRs and RFLP typing.
Detection limit Maximum (minimal ratio of fragment size Detectable mutant to wild-type Method (kb) mutations cells) DGGE, TGGE 1 Close to ?
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