To determine whether our insights were sensitive to the method used for distinguishing between classes of heterogeneous infections, we used a newly described method (classifier of tandem repeats
[ClassTR]) (8), which uses 24-loci mycobacterial interspersed repetitive units-variable number of tandem repeats
(MIRU-VNTR) data to distinguish polyclonal and clonal infections, and we compared these findings with an analysis based on the standard threshold-based approach (9).
Microsatellites, also known as simple sequence repeats (SSRs) or short tandem repeats
(STRs), are highly polymorphic and codominant molecular markers that are based on simple repeated and frequent sequences common in eukaryotic genomes, which have proven to be an effective tool in genetic diversity and molecular ecology studies (Goldstein and Schloterer, 1999).
In this gene, polymorphism of a different number of CAG tandem repeats
encoding the polyglutaminic chain of different lengths, has been identified.
Most forensic analysis focuses on short tandem repeats
(STRs) containing many alleles, or variations in DNA sequence length, to make identifications.
The findings were verified independently by identical experiments in each laboratory and were traced back to genetic differences between the two batches already at the time of purchase that were not detected in initial authenticity tests of short tandem repeats
but only by comparative genomic hybridization.
The difference between genome sizes among the four isolates are principally due to a deletion of ORFs 23/24 and 14/15 and the presence of variable numbers of tandem repeats
(VNTR) in ORFs 75, 94 and 125.
15 Variable number tandem repeats
(VNTR) typing was performed on NDM-1-positive Klebsiella pneumonia recovered from clinical samples of patients admitted to the intensive care units (ICUs) of different hospitals.
Polymorphism of variable-number tandem repeats
at multiple loci in Mycobacterium tuberculosis.
Chlamydia psittaci strain B was isolated in chicken egg embryos and typed by multilocus sequence variable number of tandem repeats
analysis, multilocus sequence typing, and ompA gene sequencing as a CP3-like strain.
According to Ahlstrom, the cards deliver an ideal solution for a wide range of applications including Polymerase chain reaction (PCR), real-time PCR and short tandem repeats
ReviSTER: an automated pipeline to revise misaligned reads to simple tandem repeats
Variable Number Tandem Repeats
(VNTRs) genotyping was performed for 30 cases in two groups of CHM (n=21) and PHM (n=9), with Polymerase Chain Reaction (PCR) amplification of 2 different polymorphic loci, namely the Col2A1 and D1580.