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After fixation, the lumbar spinal cords with posterior roots were removed en bloc and each was dissected into three gross samples (A, B and C): the transverse section with lumbar spinal cord of L3 (sample A) for light microscopic examination and TUNEL staining (see TUNEL staining for apoptosis below), the posterior corner section of L3 (sample B), and the posterior roots section of L3 (sample C) for electron microscopic examination.
The utility and relative simplicity of the TUNEL assay makes it useful for an inquirybased laboratory.
H] = helper T cell, TNF = tumor necrosis factor, TUNEL = terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate nick-end labeling.
TUNEL assays and 2-color flow cytometry were used to distinguish apoptotic hemocytes from nonapoptotic cells and to correlate apoptosis frequencies with cell cycle stage as estimated by relative cellular DNA contents (Fig.
Because most NRBCs were found in Annexin V-negative/CD71-positive fraction, only the cells from this fraction were used to perform the following TUNEL and fluorescent in situ hybridization (FISH) analyses.
Although this sweeping novel contained some of the same themes Sabato had first explored in El tunel, it also introduced some daring innovations, especially the third section, Informe sobre ciegos which, in its twisted madness, purports to demonstrate that a kind of conspiratorial evil resides in a community of the blind.
Following dermaPACE treatment, significant increases in vWF, VEGF, eNOS, PCNA and EGF expressions, plus a decrease in TUNEL expression, were demonstrated (p<0.
In situ assessment of apoptosis by TUNEL histochemistry
TUNEL staining increased in dose- and time-dependent manners in differentiating BMSCs (Figure 4).
1% Triton X-100 (Sigma Co, USA) for 5 min at 4[degrees]C and incubated with 50 [micro]l TUNEL reaction mixture in a humidified chamber at 37[degrees]C for 1 hr.
Amac: Karpal tunel sendromunda (KTS) dusuk enerjili lazer (DEL) ve kesikli ultrason (US) tedavilerinin etkinliklerini degerlendirmek ve karsilastirmaktir.
The qualitative impression of TBMEHP-induced hepatocyte apoptosis was confirmed by TUNEL staining of liver cross-sections, with high-dose dams having a significant increase in TUNEL-positive events (Figure 3E).
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