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Abbreviation for:
tectospinal tract
thermoregulatory sweat test
thromboplastin screening test
timed sequential therapy
toxic-shock syndrome toxin
transition support team  
treadmill stress test
triceps skinfold thickness
tuberculin skin test
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.


1. Toxic shock toxin.
2. Treadmill stress test, see there.
McGraw-Hill Concise Dictionary of Modern Medicine. © 2002 by The McGraw-Hill Companies, Inc.

Tuberculosis: Skin and Blood Tests

Synonym/acronym: TST, TB tine test, PPD, Mantoux skin test, QuantiFERON-TB Gold blood test (QFT-G), QuantiFERON-TB Gold In-Tube test (QFT-GIT), T-SPOT.TB test (T-SPOT).

Common use

To evaluate for current or past tuberculin infection or exposure.


Whole blood (5 mL) collected in a green-top (LiHep) tube (QuantiFERON-TB Gold and T-SPOT.TB blood tests), 1 mL whole blood collected in each of 3 special (Nil, Antigen and Mitogen) specimen containers (QuantiFERON-TB Gold In-Tube test).

Normal findings

(Method: Intradermal skin test, enzyme-linked immunosorbent assay [ELISA] blood test for QuantiFERON assays, enzyme-linked ImmunoSpot [ELISPOT] for T-SPOT.TB test) Negative.


Routine screening for tuberculosis in the U.S. has not been needed for some time because until recently the disease had largely been eradicated. Recommendations for the timing of initial and subsequent screening may vary according to state laws, practitioners’ guidelines, and specific circumstances (foreign adoptions or immigration from areas where tuberculosis is endemic, or in high risk environments such as healthcare or institutionalized settings). Children and adults are screened based on a risk assessment. For example the America Academy of Pediatrics recommends identifying individuals at highest risk by means of a questionnaire before testing, while adults who either work or reside in a high risk environment are required to submit to annual Mantoux testing or chest x-ray (for individuals with a previously positive Mantoux test or individuals from other countries who have received the BCG vaccine). Tuberculin skin tests are done to determine past or present exposure to tuberculosis (TB). The multipuncture or tine test is no longer used as a screening technique and has been largely replaced by the more definitive Mantoux test using Aplisol or Tubersol, purified protein derivatives of the mycobacterial cell wall, administered by intradermal injection. The Mantoux test is the test of choice in symptomatic patients. It is also used in some settings as a screening test. A negative result is judged if there is no sign of redness or induration at the site of the injection or if the zone of redness and induration is less than 5 mm in diameter. A positive result is evidenced by an area of erythema and induration at the injection site that is greater than 10 mm. A positive result does not distinguish between active and dormant infection. A positive response to the Mantoux test is followed up with chest radiography and bacteriological sputum testing to confirm diagnosis. The QuantiFERON-TB Gold (QFT-G), QuantiFERON-TB Gold In-Tube (QFT-GIT), and T-SPOT.TB interferon release blood tests are approved by the U.S. Food and Drug Administration for all applications in which the TB skin test is used. The blood tests are procedures in which T lymphocytes from the patient, either in whole blood or harvested from whole blood, are incubated with a reagent cocktail of peptides that simulate two or three proteins, ESAT-6, CFP-10, and TB7.7, made only by Mycobacterium tuberculosis. These proteins are not found in the blood of previously vaccinated individuals or individuals who do not have TB. The blood test offers the advantage of eliminating many of the false reactions encountered with skin testing, only a single patient visit is required, and results can be available within 24 hr. Results obtained by the QFT-G test are not affected by BCG vaccination. The blood tests and skin tests are approved as indirect tests for Mycobacterium tuberculosis, and the Centers for Disease Control and Prevention (CDC) recommends their use in conjunction with risk assessment, chest x-ray and other appropriate medical and diagnostic evaluations.

This procedure is contraindicated for



  • Evaluate cough, weight loss, fatigue, hemoptysis, and abnormal x-rays to determine if the cause of symptoms is TB
  • Evaluate known or suspected exposure to TB, with or without symptoms, to determine if TB is present
  • Evaluate patients with medical conditions placing them at risk for TB (e.g., AIDS, lymphoma, diabetes)
  • Screen populations at risk for developing TB (e.g., health-care providers [HCPs], nursing home residents, correctional facility personnel, prison inmates, and residents of the inner city living in poor hygienic conditions)

Potential diagnosis

Positive findings in:

  • Pulmonary TB

Critical findings

  • Positive results
  • It is essential that a critical finding be communicated immediately to the requesting health-care provider (HCP). A listing of these findings varies among facilities.

  • Timely notification of a critical finding for lab or diagnostic studies is a role expectation of the professional nurse. Notification processes will vary among facilities. Upon receipt of the critical value the information should be read back to the caller to verify accuracy. Most policies require immediate notification of the primary HCP, Hospitalist, or on-call HCP. Reported information includes the patient’s name, unique identifiers, critical value, name of the person giving the report, and name of the person receiving the report. Documentation of notification should be made in the medical record with the name of the HCP notified, time and date of notification, and any orders received. Any delay in a timely report of a critical finding may require completion of a notification form with review by Risk Management.

Interfering factors


  • Each of the blood and skin tests evaluate different facets of the immune response and use different methodologies and reagents; interpretations may not be interchangeable.
  • Skin Test

  • Drugs such as immunosuppressive agents or steroids can alter results.
  • Diseases such as hematological cancers or sarcoidosis can alter results.
  • Recent or present bacterial, fungal, or viral infections may affect results. False-positive results may be caused by the presence of nontuberculous mycobacteria or by serial testing.
  • False-negative results can occur if sensitized T cells are temporarily decreased. False-negative results also can occur in the presence of bacterial infections, immunological deficiencies, immunosuppressive agents, live-virus vaccinations (e.g., measles, mumps, varicella, rubella), malnutrition, old age, overwhelming TB, renal failure, and active viral infections (e.g., chickenpox, measles, mumps).
  • Improper storage of the tuberculin solution (e.g., with respect to temperature, exposure to light, and stability on opening) may affect the results.
  • Improper technique when performing the intradermal injection (e.g., injecting into subcutaneous tissue) may cause false-negative results.
  • Incorrect amount or dilution of antigen injected or delayed injection after drawing the antigen up into the syringe may affect the results.
  • Incorrect reading of the measurement of response or timing of the reading may interfere with results.
  • It is not known whether the test has teratogenic effects or reproductive implications; the test should be administered to pregnant women only when clearly indicated.
  • The test should not be administered to a patient with a previously positive tuberculin skin test because of the danger of severe reaction, including vesiculation, ulceration, and necrosis.
  • The test does not distinguish between current and past infection.
  • Blood Test

  • The performance of these blood tests has not been evaluated in large studies with patients who have impaired or altered immune function, have or are highly likely to develop TB, are younger than 17, are pregnant, or have diseases other than TB. These individuals are either immunosuppressed, immunocompromised, or have immature immune function and may not produce sufficient numbers of T lymphocytes for accurate results. The testing laboratory should be consulted for interpretation of results or limitations for use with patients in these categories.
  • False-negative results are possible due to exposure or infection prior to development of detectable immune response.
  • False-positive results are possible due to some cross-reactivity to some strains of environmental mycobacteria.
  • False-negative results are possible due to exposure or infection prior to development of detectable immune response.

Nursing Implications and Procedure

Potential nursing problems

ProblemSigns & SymptomsInterventions
Breathing (Related to productive cough; fatigue; inflammation)Tachypnea; dyspnea; orthopnea; change in the rate and depth of respirations; retractions; nasal flare; use of accessory musclesAssess and trend respiratory rate and effort; monitor for retractions, nasal flare, and use of accessory muscles; evaluate cough effectiveness; auscultate lungs for adventitious breath sounds; monitor pulse oximetry and trend results; administer prescribed oxygen therapy; prepare for possible mechanical intubation; encourage oral fluids; encourage cough and deep breathing; pace activities
Infection (Related to exposure to Mycobacterium tuberculosis)Productive cough of bloody sputum; fever with temperature spikes; positive AFB (acid fast bacillus) smear; fatigue; night sweats; weight loss; chills; lack of appetiteSend ordered sputum specimen for culture; monitor and trend temperature; monitor AFB smear results; induce sputum as ordered if patient is unable to provide one through cough; place in respiratory isolation (negative airflow); administer prescribed medications; instruct the patient to cover the mouth when coughing or sneezing; teach the patient to wash his or her hands after coming into contact with contaminated sputum; teach the patient and family the importance of wearing masks when visiting; report all confirmed TB cases to the Department of Health
Health maintenance (Related to knowledge deficit; financial or cultural barriers; lack of social support; no motivation; complexity of health-care system)Inability or failure to recognize or process information toward improving health and preventing illness with associated mental and physical effects; self-report of difficulty managing health regime; resistance to treatment; increased symptoms; chest x-ray confirmation of disease reactivationAssess for evidence of therapeutic regime noncompliance (increased productive cough, drug-resistant culture results); identify the cause of noncompliance; arrange social services consult; teach the patient about the disease process and treatment modality in understandable, culturally appropriate terms (transmission, symptoms, length of treatment, risk to others, follow-up appointments); consider referral of the continued noncompliant to a direct observation therapy program; explain the importance of taking prescribed medication; encourage smoking abstinence
Nutrition (Related to poor appetite secondary to Mycobacterium tuberculosis infection)Unintended weight loss; pale dry skin; dry mucous membranes; documented inadequate caloric intake; subcutaneous tissue loss; hair pulls out easily; self-report of no appetiteEncourage a high-calorie, high-protein diet; encourage an increased intake of oral fluids; record accurate daily weight at the same time each day with the same scale; obtain an accurate nutritional history; assess attitude toward eating; promote a dietary consult to evaluate current eating habits and best method of nutritional supplementation; monitor nutritional laboratory values such as albumin; assess swallowing ability; encourage cultural home foods; provide a pleasant environment for eating; alter food seasoning to enhance flavor; provide parenteral or enteral nutrition as prescribed


    Blood and Skin Tests

  • Positively identify the patient using at least two unique identifiers before providing care, treatment, or services.
  • Patient Teaching: Inform the patient this test can assess for a tuberculin infection or exposure.
  • Obtain a history of the patient’s complaints, including a list of known allergens, especially allergies and sensitivities to latex.
  • Obtain a history of the patient’s immune and respiratory systems, symptoms, and results of previously performed laboratory tests and diagnostic and surgical procedures. Obtain a history of TB or TB exposure, signs and symptoms indicating possible TB, and other skin tests or vaccinations and sensitivities.
  • Obtain a list of the patient’s current medications, including herbs, nutritional supplements, and nutraceuticals (see Effects of Natural Products on Laboratory Values online at DavisPlus).
  • Review the procedure with the patient.
  • Skin Test

  • Before beginning the test, ensure that the patient does not currently have TB and has not previously had a positive skin test. Do not administer the test if the patient has a skin rash or other eruptions at the test site. Inform the patient that the procedure takes approximately 5 min. Address concerns about pain and explain that a moderate amount of pain may be experienced when the intradermal injection is performed.
  • Emphasize to the patient that the area should not be scratched or disturbed after the injection and before the reading.
  • Blood Test

  • Inform the patient that specimen collection takes approximately 5 to 10 min. Address concerns about pain and explain that there may be some discomfort during the venipuncture.
  • Blood and Skin Tests

  • Sensitivity to social and cultural issues, as well as concern for modesty, is important in providing psychological support before, during, and after the procedure.
  • Note that there are no food, fluid, or medication restrictions unless by medical direction.


  • Potential complications: N/A
  • Observe standard precautions, and follow the general guidelines in Patient Preparation and Specimen Collection. Positively identify the patient.
  • Blood and Skin Tests

  • Avoid the use of equipment containing latex if the patient has a history of allergic reaction to latex.
  • Instruct the patient to cooperate fully and to follow directions. Direct the patient to breathe normally and to avoid unnecessary movement.
  • Skin Test

  • Have epinephrine hydrochloride solution (1:1,000) available in the event of anaphylaxis.
  • Cleanse the skin site on the lower anterior forearm with alcohol swabs and allow to air-dry.
  • Multipuncture Test

  • Remove the cap covering the tines and stretch the forearm skin taut. Firmly press the device into the prepared site, hold it in place for 1 sec, and then remove it. Four punctures should be visible. Record the site, and remind the patient to return in 48 to 72 hr to have the test read. At the time of the reading, use a plastic ruler to measure the diameter of the largest indurated area, making sure the room is sufficiently lighted to perform the reading. A palpable induration greater than or equal to 2 mm at one or more of the punctures indicates a positive test result.
  • Mantoux (Intradermal) Test

  • Prepare PPD or old tuberculin in a tuberculin syringe with a short, 26-gauge needle attached. Prepare the appropriate dilution and amount for the most commonly used intermediate strength (5 tuberculin units in 0.1 mL) or a first strength usually used for children (1 tuberculin unit in 0.1 mL). Inject the preparation intradermally at the prepared site as soon as it is drawn up into the syringe. When properly injected, a bleb or wheal 6 to 10 mm in diameter is formed within the layers of the skin. Record the site, and remind the patient to return in 48 to 72 hr to have the test read. At the time of the reading, use a plastic ruler to measure the diameter of the largest indurated area, making sure the room is sufficiently lighted to perform the reading. Palpate for thickening of the tissue; a positive result is indicated by a reaction of 5 mm or more with erythema and edema.
  • Blood Test

  • Observe standard precautions, and follow the general guidelines in Patient Preparation and Specimen Collection. Positively identify the patient, and label the appropriate specimen container with the corresponding patient demographics, initials of the person collecting the specimen, date, and time of collection. Perform a venipuncture.
  • Remove the needle and apply direct pressure with dry gauze to stop bleeding. Observe/assess venipuncture site for bleeding or hematoma formation and secure gauze with adhesive bandage.
  • Promptly transport the specimen to the laboratory for processing and analysis.


  • Inform the patient that a report of the results will be made available to the requesting HCP, who will discuss the results with the patient.
  • Recognize anxiety related to test results and be supportive of perceived loss of independence and fear of shortened life expectancy. Discuss the implications of abnormal test results on the patient’s lifestyle. Provide teaching and information regarding the clinical implications of the test results, as appropriate. Counsel the patient, as appropriate, regarding the risk of transmission and proper prophylaxis, and reinforce the importance of strict adherence to the treatment regimen. Inform the patient that positive findings must be reported to local health department officials, who will question him or her regarding other persons who may have been exposed through contact. Educate the patient regarding access to counseling services.
  • Depending on the results of this procedure, additional testing may be performed to evaluate or monitor progression of the disease process and determine the need for a change in therapy. Evaluate test results in relation to the patient’s symptoms and other tests performed.
  • Patient Education

    • Reinforce information given by the patient’s HCP regarding further testing, treatment, or referral to another HCP.
    • Emphasize to the patient who receives skin testing the need to return and have the test results read within the specified time frame of 48 to 72 hr after injection.
    • Inform the patient that the effects from a positive response at the site can remain for 1 wk.
    • Educate the patient that a positive result may put him or her at risk for infection related to impaired primary defenses, impaired gas exchange related to decrease in effective lung surface, and intolerance to activity related to an imbalance between oxygen supply and demand.
    • Answer any questions or address any concerns voiced by the patient or family.
  • Expected Patient Outcomes

    • Knowledge
    • States understanding of the importance of covering the mouth during coughing or sneezing to decrease possibility of droplet contamination
    • States understanding of the importance of an adequate fluid intake to liquefy secretions
    • Skills
    • Demonstrates the correct technique for covering the mouth and nose when coughing or sneezing
    • Demonstrates proficient cough and deep breathing
    • Attitude
    • Complies with the request to refrain from smoking
    • Complies with the recommendation to take prescribed medication as ordered

Related Monographs

  • Related tests include alveolar/arterial gradient, angiography pulmonary, biopsy lung, blood gases, broncho-scopy, calcium, carbon dioxide, chest x-ray, CBC WBC count and differential, CT thoracic, culture and smear mycobacteria, culture blood, culture sputum, cytology sputum, eosinophil count, ESR, gallium scan, Gram stain, lung perfusion scan, lung ventilation scan, mediastinoscopy, pleural fluid analysis, PFT, and zinc.
  • Refer to the Immune and Respiratory systems tables at the end of the book for related tests by body system.
Handbook of Laboratory and Diagnostic Tests, © 2013 Farlex and Partners
References in periodicals archive ?
Hospitality Props Tst is scheduled to hold a conference call at 10:00 a.m.
Since the contract awarding in 2014, TST has worked closely with Shanghai Shentong Metro Group Co., Ltd.
Previously, Schwartzman and Menzies (42) examined the idea of preimmigration TST screening in addition to standard preimmigration chest radiograph coupled with postarrival isoniazid treatment.
All the patients had to have a previous negative TST test ([less than or equal to]5 mm) prior to the beginning of the first biological treatment.
Individuals who did not consent, administered TST recently, underwent TB in the past or had suspicion of TB, lived in the same house with a TB patient recently, diagnosed with cancer and/or were currently receiving therapy, with autoimmune and immunosuppresive diseases were excluded from the study.
Variables that could be associated with TST and QFT-GIT results were as follows: sex, age, presence of epidemiological risk factors for LTBI, X-ray findings indicative of LTBI, immunosuppressive therapy, and inflammatory disease activity at the time of performing the test were included in the exact logistic regression analysis, including variables with p<0.10 in the univariate analysis.
TST and QFT-IT presented regular agreement (kappa = 0.34), with similar results when stratified by IFNG + 874 A/T polymorphism genotypes (0.33 for the AA genotype and 0.28 for the TT/TA genotype).
Because the main objective of this study was to compare the distributions of the TST results, assessments of the results in terms of "positive" or "negative" were not done.
* For individuals at high risk of infection but not at high risk of disease progression, IGRA is recommended if they have received a bacille Calmette-Guerin vaccine or are unlikely to return for TST interpretation.
In this case, the organ donor risk assessment questionnaire administered to family members included questions about TB risk factors, but family members were not aware of or did not recall the donor's previous positive TST result.
ITEM: The TsT, passed recently by the House, includes two minor (in revenue impact) tax measures: one that will reduce and restructure the donor's tax on net donations for gifts exceeding P100,000, and another that will reduce and restructure the estate tax based on the net value of the estate.