It has been shown in mice that conditional deletion of Trf1 or Trf2
in type 2 alveolar epithelial cells leads to a significant telomere damage response, cellular senescence, and a pulmonary fibrosis phenotype, while maintaining normal telomere length [5, 32].
This involves activation of ATM, which is normally suppressed by TRF2
and/or activation of ATR which is normally suppressed by POT1 .
and GAPDH are both involved in the regulation of telomere homeostasis, the aim of this study was to determine if there was a correlation between the expression of these genes in primary cell cultures of ovarian surface epithelium, using qRT-PCR.
Step 3 : (trf1*trf1) [right arrow] [freel1] [right arrow] (trf2
) [right arrow] [freel2] [right arrow] [*trf1@]
The investigators synthesized telomeric DNA and mixed it with TRF1 or TRF2
. Viewed under an electron microscope, telomeres exposed to TRF2
often displayed large circles, which the researchers named t loops, but no single-stranded ends.
They are telomere repeat-binding factor 1 (TRF1), telomere repeat-binding factor 2 (TRF2
), repressor-activator protein 1 (RAP1), TRF1- and TRF2-interacting nuclear protein 2 (TIN2), tripeptidyl-peptidase 1 (TPP1), and protection of telomere 1 (POT1) .
Liu, "The protein network surrounding the human telomere repeat binding factors TRF1, TRF2
, and POT1," PLoS ONE, vol.
Unipex Innovations; and "Role of TRF2
in Preventing Cellular Senescence and Associated Skin Damages,"Isabellc Imbert, Ph.D., J.M.
. Curso de Direito Avancado de Direito Privado, EMARF, TRF2
: ACOES POSSESSORIAS (caso Jardim Botanico) E USUCAPIAO DE BENS PUBLICOS; Palestrantes: Poul Erik Dyrlund e Miriam Fontenelle; em 29 de Junho de 2009;
, Apelacao/Reexame Necessario 612631, Rel.
De Lange, "p53and ATM-dependent apoptosis induced by telomeres lacking TRF2
," Science, vol.