Effects ofMEKT1 on the Interaction between Nur77/Nurr1 and NurRE, Tpit and TpitRE, and Nur77 and NBRE on the Pomc Promoter.
Moreover, it is already established that Nur77/Nurr1, NeuroD1, Tpit, Pitx, NFkB1, and NFkB2 are important transcription factors for Pomc expression [25, 26, 33, 37-40].
To verify the importance of transcriptional factors Nur77, Nurr1, and Tpit, we also performed an overexpression experiment and observed that the MEKT1-mediated suppression of Pomc promoter activity was attenuated by the Nur77 and Tpit overexpression (Figure 9).
We can conclude as shown in Figure 11 that Nur77/Nurr1 heterodimer binding element NurRE (-383/-404), Tpit responsive element TpitRE (-309/-316), and Nur77 monomer responsive element NBRE (-63/-69) play important roles in Pomc expression [32, 33, 40].
Figure S3: MEKT1mediated effects on the mRNA expression of Nur77, Nurrl, and Tpit in AtT20 cells are dose-dependent.
Lamonerie et al., "A pituitary cell-restricted T box factor, Tpit, activates POMC transcription in cooperation with Pitx homeoproteins," Cell, vol.
Caption: FIGURE 7: MEKT1-mediated effects on the mRNA expression of mouse Nur77, Nurrl, NeuroDl, Tpit, Pitx, NFkBl, and NFkB2 in AtT20 cells.
Caption: FIGURE 8: Effects of MEKT1 (time dependently), rosiglitazone, and pioglitazone on Nurrl, Nur77, and Tpit protein expression.