TPHA


Also found in: Dictionary, Thesaurus, Legal, Financial, Acronyms, Encyclopedia, Wikipedia.
Related to TPHA: bilharziasis, RPR

assay

 [as´a]
determination of the purity of a substance or the amount of any particular constituent of a mixture.
biological assay bioassay; determination of the potency of a drug or other substance by comparing the effects it has on animals with those of a reference standard.
CH50 assay a test of total complement activity as the capacity of serum to lyse a standard preparation of sheep red blood cells coated with antisheep erythrocyte antibody. The reciprocal of the dilution of serum that lyses 50 per cent of the erythrocytes is the whole complement titer in CH50 units per milliliter of serum.
enzyme-linked immunosorbent assay (ELISA) any enzyme immunoassay using an enzyme-labeled immunoreactant (antigen or antibody) and an immunoadsorbent (antigen or antibody bound to a solid support). A variety of methods are used for measuring the unknown concentration, such as either competitive binding between the labeled reactant and unlabeled unknown or a sandwich technique in which the unknown antigen binds both the immunoadsorbent and labeled antibody. One of the uses of ELISA is to screen blood for antibody to the human immunodeficiency virus; a positive result indicates probable exposure to the virus and possibly that the virus is in the blood. Since false-positives can occur, a back-up test is used to confirm positive findings.
 ELISA: With antigen capture ELISA, the wells are coated with antibody to the virus. The sample containing virus (1) is added and, after washing several times, enzyme conjugated to an antibody to the virus is added (2). Finally, after a further cycle of washing, the enzyme's substrate (3) is added. A colored product is formed if the viral antigen is present in the sample (4). From Hart and Shears, 1997.
microhemagglutination assay–Treponema pallidum (MHA-TP) a Treponema pallidum hemagglutination assay using microtechniques; used in the detection of syphilis.
radioreceptor assay a radioligand assay in which a radiolabeled hormone is used to measure the concentration of specific cellular receptors for the hormone in tissue specimens, an example being radioassay of estrogen receptors in breast tissue.
thyroid-stimulating hormone assay thyroid-stimulating hormone test.
Treponema pallidum hemagglutination assay (TPHA) a treponemal antigen serologic test for syphilis using tanned sheep red blood cells coated with antigen from the Nichol's strain of Treponema pallidum and treated patient serum; it is similar in sensitivity and specificity to the FTA-ABS test. This test is not useful for individuals who have had syphilis in the past.

TPHA

abbreviation for Treponema pallidum hemagglutination assay.
References in periodicals archive ?
After analysis with other treponemal and nontreponemal tests, and when evaluated together with patients' history of STD, three samples were interpreted as probable false-negative TPHA results, implying that the CIA may have been more sensitive in detecting latent syphilis than the TPHA.
Treponemal antigen based enzyme immunoassays (EIAs) are an appropriate alternative to the combined VDRL/RPR and TPHA screen7.
TPHA was also recognized for giving out $500 "Visionary Awards" that recognize Tennesseans who might not work in public health but encourage healthier lifestyles, Levy said.
The aims of this study were to determine and compare the prevalence of syphilis among different patient groups at Weskoppies Hospital, to determine and compare the psychiatric diagnoses of the infected and non-infected, to assess self-reported high-risk sexual behaviour, to establish the co-morbidity between syphilis and HIV, and to investigate the performance of the non-treponemal RPR test as screening method, compared with the confirmatory TPHA test.
Besides, the prevalence of HIV infection was significantly higher among those with positive TPHA and HSV-2 results.
Though the patient had high titre positivity for VDRL and TPHA, histopathology did not reveal the presence of plasma cells or features of endarteritis obliterans.
determining the object and the size or scope of the order: Delivery of enzyme immunoassay and immunochromatography, TPHA reagents, reagent Syphilis IgM EIA Assay, antigens, substrates, sheep blood, testing for genetic determination of antigens, kits for diagnosis of allergies - 15 packages described in detail Annex No.
Investigational reports like Grams stain and culture, FNAC, tissue smear, urethral smear, Tzanck smear, KOH, wet mount preparations, chest X-ray along with serological tests like ELISA, Rapid tests for HIV, RPR, VDRL and TPHA for syphilis were noted.
In the meantime VDRL TPHA HBsAg HCV antibodies and Toxoplasma IgG were done and were all negative.
VDRL (Venereal Disease Research Laboratory) and TPHA (Treponema pallidum hemagglutination assay) results were nega-tive.