Cyclo-oxygenase-2 (COX-2), transforming growth factor-alpha (TGF alpha
) and heparin-binding epidermal-growth-factor-like growth factor (HB-EGF) mRNA and protein expression were assessed by RT-PCR and Western blot analysis, respectively; malondialdehyde (MDA) was determined by HPLC; gastric secretion was evaluated in pylorus-ligated rats.
Activation of hepatic stellate cells by TGF alpha
and collagen type 1 is mediated by oxidative stress through c-myb expression.
"TGF alpha has been studied for two decades in other organ systems but never before has been shown to reverse the symptoms of a stroke," Guerra-Crespo said.
Scientists sought to learn whether TGF alpha administered directly to the brain could help rats with stroke-induced loss of limb function, typically on one side - as is seen in humans.
Methods are provided for inhibiting the differentiation of adipocyte precursor cells, and for treating or preventing obesity, which comprise administering an effective amount of a composition capable of binding to and activating the EGF receptor, preferably epidermal EGF or a functional derivative thereof, TGF Alpha
, an antibody specific for the EGF receptor or an anti-idiotypic antibody specific for an idiotope on an antibody specific for EGF.