PRCV has a 224-aa deletion at positions 21-244 in the N terminal region, which is needed for the enteric tropism of TGEV
and comprises antigenic sites C and B (13-15), of S1 compared with TGEV
The company has been able to demonstrate the efficacy of its TGEV vaccine in clinical trials.
To acquire the TGEV patent, ProdiGene was able to demonstrate the ability of its oral vaccine to overcome a major obstacle for many oral vaccines, the inability of the antigen to survive in the gut thereby neutralizing the body's ability to mount an immune response.
Of the 89 PDCoV-positive samples, 20 samples (22% [11 fecal, 6 intestinal homogenate, 2 fecal swab, and 1 feed]) were negative for TGEV, porcine epidemic diarrhea virus (PEDV), rotavirus A (RVA), rotavirus B (RVB), and rotavirus C (RVC); 69 (78%) PDCoV-positive samples were positive for PEDV, RVA, RVB, or RVC.
We identified positive PDCoV in 20 (22%) samples that were negative for TGEV, PEDV, RVA, RVB, and RVC, but PDCoV co-infections were more common (69 [78%] samples), especially with RVC (52 [58%]).
Protection from TGEV proves that this technology is an innovative method to address animal and human health at a time when fewer therapeutics, such as antibiotics, are available," said Joe Jilka, Ph.
Value is added to both livestock producers and grain farmers who will contract to grow the grain-based vaccines," said Dan Hammes, ProdiGene vice president of operations, who added that the preliminary trials used corn enhanced to create the protein that stimulates the TGEV antibody.
gt;96% aa identity in the key replicase 1ab domains), TGEV
, FCoV, and CCoV should not be considered separate viruses.
Relative to pathogenesis, 5 informative amino acid sites were found in the membrane protein at positions 108, 120, 138, 163, and 199 (based on reference sequence for TGEV
The virus-serum mixtures were then added to 96-well microtiter plates (Costar) containing overnight confluent monolayers of Vero V76 cells or PT-K75 cells, for the SARS or TGEV
CPE-blocking assay, respectively.
Immunization with vaccines made from other members of the feline coronavirus group, TGEV
or canine coronavirus, also sensitizes cats to early death syndrome (39).
No specific neutralization was observed when polyvalent or monoclonal antibodies that neutralized TGEV
or the human coronaviruses OC43 or 229E were used in a standard neutralization assay.