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La identificacion molecular se llevo a cabo mediante el analisis de las secuencias de los espaciadores transcritos internos 1 y 2 del ADN ribosomal (ADNr) y parte del gen del factor de elongacion de la traduccion 1[alfa] (tef1).
gattii MLST scheme (CAP10, GPD1, IGS1, LAC1, MPD1, PLB1, and TEF1) (12).
Overexpression of GSH1 and GSH2 was driven by the strong Hc Tef1 promoter.
CO1 distinguished only two species, while all six were resolved by each of the other two markers--called translation elongation factor 1-alpha (Tef1) and internal transcribed spacer of ribosomal RNA gene (ITS-rDNA).
The genetic variability within 69 biocontrol isolates of Trichoderma collected from different geographic locations and culture collections and their phylogenetic analysis were done with the help of the sequence data obtained from the inter Transcribed spacer 1(ITS1) region of Ribosomal DNA and a fragment of the translation elongation factor 1(tef1) and reported that more than 50% of the potential bio-control strains were grouped within Trichoderma section Pachybasium reported by Hermosa, M.
Interactions between the most closely related proteins were also identified and assigned values; these proteins included SPE1, ARF1, HIS3, AMS1, HHT1, TEF1, and TOM1.
The MLST loci CAP10, CAP59, GPD1, IGS, LAC1, MPD1, PLB1, SOD1, TEF1 a, and URA5 of the environmental C.
La comparacion de secuencias determino varias posibilidades de identificacion en TVC36 y TVC56, debido a que las regiones ITS no aportan suficiente informacion para dar una identificacion definitiva, por lo que requiere el estudio de otras secuencias como el factor de elongacion de la transcripcion l[alfa] (tef1) para completar la identificacion (Druzhinina et al., 2006).
Forced expression of MS8 in the yeast form was accomplished by transforming cells with a vector carrying MS8 fused to the strong constitutive Tef1 promoter.
Our results indicate that genetic material from AFLP1/VNI and AFLP4/VGI was present in CBS10496, although only 1 allele was obtained for TEF1 [alpha] and ITS.
The genetic variability within 69 biocontrol isolates of Trichoderma collected from different geographic locations and culture collections and their phylogenetic analysis were done with the help of the sequence data obtained from the ITS-1 region of ribosomal DNA and a fragment ofthe translation elongation factor 1 (tef1) and reported that more than 50% of the potential biocontrol strains were grouped within Trichoderma section Pachybasium (21,22).
For MLST analysis, DNA fragments of 8 unlinked genes that included CAPIO, GPD, IGS1, LAC1, PLB1, SOD1, TEF1, and URE1, were amplified by PCR (10) from 6 randomly chosen C.