In order to develop a protective immune response goats must be inoculated with PPRV vaccine having 103 TCID50
or higher virus concentration.
These vaccines were (9) BEI- AHG -FMDV with 10-6 units of TCID50
F, 4-8degC, (10) BEI- AHG -FMDV with 10-6 units of TCID50
2M, 4-8 0C, 11 -BEI- AHG -FMDV with 10-6 units of TCID50
4M, 4-8 0C and 12-BEI- AHG -FMDV with 10-6 units of TCID50
To prepare gel based vaccine sterile aluminium hydroxide gel was added at a final concentration of 10 % in the PPR virus suspension with a titer of 104 TCID50
Fifty microliter of each dilution of each serum sample was admixed with equal volume of 100 units of TCID50
of "O" serotype of FMD virus and the filtered mixture was transferred on to respective well containing monolayer of BHK-21 cell line in 96 welled cell culture plates, aseptically.
07 units of virus TCID50
in the stationary monolayer of BHK-21 cells in roux flask (75cm2), log107.