These organisms are the causative agents of the neglected diseases; African sleeping sickness in sub-Saharan Africa (T. brucei
) and Chagas disease in Central and South America (T.
In central and west Africa, Trypanosoma brucei gambiense causes the slow-progressing form of the disease, and T. brucei
rhodesiense causes the fast progressing form in east and southern Africa (1).
The Human African Trypanosomiasis (HAT) is caused by two subspecies of trypanosomes, namely, Trypanosoma brucei gambiense which is prevalent in west and central Africa and causes chronic disease and T. brucei
rhodesiense which occurs in East and southern Africa and causes acute form of disease.
This disease is transmitted by tsetse fly (genus Glossina), a vector which is only found in tropical Africa, this explains the T. brucei
geographic restriction [5, 6].
Out of 283 blood samples, 19 (6.71%) generated a 164 bp DNA fragment specific for T. brucei
. Only 7 blood samples (2.5%) were found Trypanosome sp.
Therefore, in T. brucei
brucei-infection with increased ROS production, the immune cells are vulnerable to oxidative damage, which may be responsible, among other factors, for the leucopenia observed in the infected untreated group.
In the case of gene products called tRNAs, which help assemble the amino acids that make proteins, T. brucei
was known to have only one tRNA with a specific segment of RNA that ensures the tRNA's proper function.
(1989) where they obtained excellent growth during the culture of Trypanosoma brucei brucei and T. brucei
The life cycle of T. brucei
subspecies begun in human infected with metacyclic forms then differentiate into bloodstream, subcutaneous tissues and lymph (Sternberg 2004).
17-AAG could likely be effective against infection caused by T. brucei
due to the sequence similarity between TbHsp90 and TeHsp90 .
brucei, the cause of Nagana, was chosen as the model for this study because it mimics HAT, which is caused by T.b.
Two subspecies are responsible for human disease; T. brucei
rhodensiense in East Africa and T.