Adrenal responses to a low-dose short synacthen
test in children with asthma.
ii) Adrenocorticotropic hormone administration: A standardised dose (1 [micro]g/kg), of ACTH (Synacthen
Depot, tetracosactide 1 mg/mL, Lot S1358, Novartis, South Africa) was administered once to each of the six cows at time 0 .
Based on this evaluation, further biochemical assessments including synacthen
test, ACTH, renin and detailed plasma steroid measurements by LC-MS/MS were undertaken when necessary and only for the cases suggestive of CAH.
test showed cortisol levels of 129 nmol/L, 134 nmol/L and 151 nmol/L at 0 minutes, 30 minutes and 60 minutes, respectively.
Adrenocorticotropic hormone (ACTH) stimulation was used during all the procedures: 750 [micro]g of ACTH (Synacthen
; CD Pharma AB) was diluted in 800 mL of 0.9% saline, which was infused at a rate of 100 mL/h (94 [micro]g/h), starting two hours prior to the procedure.
Serum cortisol/cortisone ratio after Synacthen
Stimulation test was done by intravenous bolus injection of 0.25 mg 1-24 ACTH (Synacthen
; Ciba/Novartis, Basel, Switzerland), and venous blood samples were collected 30 and 60 min later for the measurement of cortisol and 17-OH-P concentrations.
McCance et al., "Comparison of one week 0900 h serum cortisol, low and standard dose synacthen
tests with a 4 to 6 week insulin hypoglycaemia test after pituitary surgery in assessing HPA axis," Clinical Endocrinology, vol.
Hyperprolactinaemia (927 mU/L [45-375]) and secondary hypothyroidism (FT4: 7.3 pmol/L [9.4-24.0], TSH: 0.7 mU/L) were also noted, and secondary adrenal failure was excluded by a short Synacthen
test (baseline cortisol: 223 nmol/L, peak: 467 nmol/L).
Stewart, "The long-term predictive accuracy of the short synacthen
(corticotropin) stimulation test for assessment of the hypothalamic-pituitary-adrenal axis," Journal of Clinical Endocrinology and Metabolism, vol.
However, upon performing a Synacthen
stimulation test, the adrenal axis was found to be still within normal functioning.
(ACTH stimulation test) was abnormal at 1.76 ug/dl, range (2.5-10.5).