The deficiency of PA depletes the concentration of CoA, which reduces the conversion to succinyl-CoA synthetase
and glycine as the first step in the synthesis, subsequently reducing the concentration of hemoglobin in the blood (Depeint et al., 2006).
The 5 proteins with increased expression levels were identified as type II peroxiredoxin 1, ATP-specific succinyl-CoA synthetase beta subunit, rho GDP-dissociation inhibitor 1, heme-binding protein, and phosphatidylethanolamine-binding protein 1, and the 11 proteins with decreased expression levels were identified as profilin-2, tubulin beta-5 chain, alpha-internexin, ketimine reductase mu-crystallin, L-lactate dehydrogenase B chain isoform 1, ubiquitin carboxy-terminal hydrolase L1, isoform CRA_a, proteasome subunit alpha type-3, N(G),N(G)-dimethylarginine dimethylaminohydrolase 1, ferritin heavy chain, translationally controlled tumor protein, and prohibitin (Table 1).
Western blot analysis was performed to validate the identity of translationally controlled tumor protein and ATP-specific succinyl-CoA synthetase beta subunit as differentially expressed hippocampal proteins and the identity of gamma-enolase isoform 1 as differentially expressed cortical protein.
Caption: Figure 3: Representative images of Western blotting evaluating the expression of translationally controlled tumor protein (TCTP) (a), ATP-specific succinyl-CoA synthetase beta subunit (SUCLA2) (b) in the hippocampus, and gamma-enolase isoform 1 (NSE) (c) in the cortex from STZ-treated (STZ) and control (CTL) mice.
However, an ADP-dependent succinyl-CoA synthetase activity was purified from Thermococcus kodakarensis, a member of the Thermococcales and a close relative of P.
Imanaka, "A novel ADP-forming succinyl-CoA synthetase in Thermococcus kodakaraensis structurally related to the archaeal nucleoside diphosphate-forming acetyl-CoA synthetases," The Journal of Biological Chemistry, vol.
Homologs of ACS, known as succinyl-CoA synthetases (SCS), have been characterized from eukaryotes as well as bacteria [32, 33].