Bacterial families which were present in two tissues with biofilms were Alteromonadaceae, Propionibacteriaceae, Streptomycetaceae
, Streptococcaceae, Enterobacteriaceae, Comamonadaceae, and Flavobacteriaceae and may thus be interesting candidates for further analysis as initiators of chronic wound infection in dogs.
BLAST result of the nearly complete 16S rRNA gene sequences (averaging 1,529 nucleotides) of the strain BTSS 1001 against sequences in the GenBank database revealed homologies of 99% to members of the family Streptomycetaceae
. The BLAST result gave similarity matches to Streptomyces rochei (99.00%), Streptomyces plicatus (99.00%), Streptomyces olivaceus (99.00%), and Streptomyces enissocaesilis (99.00%).
The PCA plot indicates that Micromonosporaceae, Streptomycetaceae
, and Dactylosporaceae are closely grouped while their distribution was influenced by alkalinity and sulphate concentration while Thermonosporaceae and Streptosporangaeae are grouped separately and their distribution is influenced by pH, potassium, sodium, and calcium ions concentration.