streptavidin


Also found in: Wikipedia.

strep·ta·vi·din

(strep'ta-vī'din),
A bacterial protein used as a probe in immunologic assays because of its strong affinity and specificity for biotin; streptavidin is used as a bridge to link a chromogen to a biotinylated substrate specific for the substance of interest.
[streptococcus + avidin]

streptavidin

[strep′təvī′din]
a biotin-binding protein isolated from Streptomyces and used to identify antigens; a widely used reagent for immunoassays.

strep·ta·vi·din

(strep-tav'i-din)
A bacterial protein used as a probe in immunologic assays because of its strong affinity and specificity for biotin; used as a bridge to link a chromogen to a biotinylated substrate specific for the substance of interest.
[streptococcus + avidin]

streptavidin

a protein found in Streptomyces griseus which has a high affinity for biotin and is used in immunofluorescent studies.
References in periodicals archive ?
Researchers found that microbubbles conjugated to streptavidin can be coupled to the surface of a cell, where mechanosensitive Piezo1 ion channels are expressed.
Once this bridge is formed, streptavidin peroxidase attached to the colorogenic substrate (TMB) is bound.
Louis, MO, USA), MG-l32 from Enzo Life Sciences (Farmingdale, NY, USA), Cy5-conjugated Streptavidin from Jackson ImmunoResearch Laboratories (West Grove, PA, USA), and Coomassie Stain Solution from Bio-Rad (Hercules, CA, USA).
This method employed a mouse monoclonal antibody (NCL-COX-2) of Vision Biosystems, NovocastraTM as primary antibody and secondary antibody of Ultra Tech HRP (DAB) Streptavidin Biotin Detection System from Beckman Coulter was used.
Possible solutions may require removal of biotin in the assay design by pretreating samples with streptavidin or by adding biotin extraction steps.
Four subprojects have been tailored to address the challenges: i) knock-out deleterious components present in the periplasm; ii) improve the cofactor uptake through the outer-membrane; iii) engineer streptavidin to boost the AMs performance; and iv) rely both on screening and selection strategies to evolve AMs in vivo.
Thermo Scientific has launched its next-generation MSIA Streptavidin EVO microcolumns for large molecule bioanalysis.
Blots were incubated with HRP-conjugated anti-rabbit secondary antibodies (1:5,000, Southern Biotech) or HRP-conjugated streptavidin (1:40,000, Invitrogen) for 1 hr at 25[degrees]C.
The money enables Quad to launch its MagCloudz Streptavidin Cell Separation Kit, a commercially available product incorporating QuickGel technology.
The plates were coated with 100 pl of Streptavidin (5 [micro]g/ml) per plate.
The DNA fragments hybridized to biotinylated probes were selectively captured by streptavidin coated magnetic beads (Streptavidin Magnesphere Paramagnetic Particles, Promega, Shanghai, China).
The leftover, unbound polymer is then added to the etalons, where the corresponding colour change indicates how much polymer is left, and in turn, how much streptavidin there was to bind with.