The corresponding value of the sample was read from the standard curve
The quantitative properties of qPCR are based upon running unknown samples alongside a standard curve
of known copy numbers of the target DNA (Larionov et al.
Because this information was not provided by Diasorin, we created our own standard curve
Virus titers were extrapolated from RT-PCR results by comparison with a standard curve
generated from serial dilutions of a VEEV stock quantified by plaque assay.
A standard curve
was included on each 96-well dish consisting of several concentrations of cells (0, 1 x [10.
2] with catalase was determined from a standard curve
Absorbance was measured at 420 nm after 30 min and plotted against concentration to made standard curve
, from where unknown concentration of phosphorous was determined (Table-4).
The beads are run on the cytometer on the same day and at the same instrument settings as cells to generate a standard curve
relating channel value to a standardized fluorescence-intensity unit--molecules of equivalent soluble fluorochrome (MESF).
coli RNA to create a standard curve
for each nucleic acid (i.
Quantification was performed using a standard curve
prepared by serial dilutions of recombinant ZAG added to 50 g/L BSA in phosphate-buffered saline as a surrogate serum matrix.
This allowed the researchers to use advanced analysis techniques to search for the genes that seemed to be sensitive to aging, and create a standard curve
, or timeline, that described the way they changed.
The standard curve
of flavonoids content was analyzed using rutin as the standard at 400 nm, using an ultraviolet (UV) spectrophotometer, and the standard curve
was obtained, C = 0.