Proteins that are competing for binding with full-length E-proteins include negative bHLHs, such as MSC, and the splice form of the full-length E-protein, E2A-2/5.
The splice form of the E2A protein, the E2A-2/5 splice variant, also competes with positive and negative bHLHs alike for binding with the full-length E2A protein.
Evidence that public database records for many cancer-associated genes reflect a splice form
found in tumors and lack normal splice forms
For example, a study examining the effects of alternative splicing on transcripts encoding membrane proteins revealed that a common splice form leads to the removal of the transmembrane domain of single-pass transmembrane proteins, producing a soluble protein isoform (30).
Furthermore, recent reports indicate that 10-30% of alternatively spliced human genes possess tissue-specific splice forms (6) and that at least 300 genes possess cancer-specific mRNA variants (7).
The very large portfolio of neurexin alternative splice forms
is strategically positioned to play an important role in synaptic plasticity.
The technology's single primer strategy requires only one partner exon to be targeted, thus allowing identification of known fusion events and discovery of new gene fusions and alternative splice forms
The AgDscam gene has 101 protein-coding regions, called exons, that can be spliced together in different combinations to produce over 31,000 possible splice forms
that function as receptors.
An important feature of alternative gene splicing is that many of the "splice variants" or "alternative splice forms" that result are specific to human disease and produce proteins that are essential to the disease process.
Founded in 2002, Alteris is an emerging biopharmaceutical company focused on the discovery and development of therapeutics and vaccines based on alternative gene splice forms unique to cancer.
The research team utilised quantitative real-time polymerase chain reaction to evaluate the expression of telomerase reverse transcriptase (TERT), splice forms
and telomere length, while the activity of telomeres was assessed by enzyme-linked immunosorbent assay (ELISA).
Alternative splicing is largely responsible for the proteomic complexity of the approximately 30,000-40,000 genes in the human genome; 40-60 percent of human genes have alternative splice forms
. Defects in the splicing process play a central role in many human diseases.