They are embedded in a hyaline capsule which has club-like projections called Splendore-Hoeppli phenomenon
Five years after the patient's death, histopathological reexamination suggested the diagnosis of basidiobolomycosis based on the presence of eosinophilic and giant multinucleated cells mixed with septate hyphal fragments and the Splendore-Hoeppli phenomenon. We submitted frozen tissue samples for PCR at the BCCM/IHEM culture collection for biomedical fungi at the Scientific Institute of Public Health in Belgium, but these were negative for B.
Histopathological results after HE staining of all three cases showed tuberculoid granulomata with giant cells (Figure 2(a)), numerous lymphocytes, histiocytes and eosinophilic cells (Figure 2(b)), an amorphous eosinophilic material also known as the "Splendore-Hoeppli phenomenon" (Figure 2(c)), and 10 [micro]m diameter septate hyphal fragments (Figure 2(b)), confirmed also by the Gomori-Grocott staining (Figure 2(d)).
Central area of the cystic spaces contain granular, basophilic, filamentous material surrounded by Splendore-Hoeppli Phenomenon
along with neutrophilic microabscesses suggestive of botryomycosis [Fig.
H&E is a versatile stain that enables the pathologist to evaluate the host response, including the Splendore-Hoeppli phenomenon
, and to detect other microorganisms.
Fungal hyphae associated with a Splendore-Hoeppli phenomenon
were visible in the left leg muscle.
Zygomycosis can be distinguished from infections caused by mucoraceous fungi by a lack of vascular invasion or infarction and the production of a prolific chronic inflammatory response and Splendore-Hoeppli phenomenon
around the hyphae.
Among these areas were numerous broad, thick septate fungal hyphae surrounded by eosinophilic proteinaceous material (Splendore-Hoeppli phenomenon
) (Figure 3).
Histopathologic evaluation of the mass revealed marked inflammation and the Splendore-Hoeppli phenomenon
, in which eosinophils are deposited around the fungus.
ranarum in tissues is characterized by scarce, broad, thin-walled, pleomorphic hyphae surrounded by a collar of eosinophilic material (known as the Splendore-Hoeppli phenomenon
is found in association with such infections as (Figure 2): [FIGURE 2 OMITTED] a.
The Splendore-Hoeppli phenomenon
describes the eosinophilic, pseudomycotic structures composed of necrotic debris and immunoglobulin.