References in periodicals archive ?
Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media.
Briefly, spirochaetal culture was centrifuged at 12,000 A- g for 10 minutes, followed by a washing of pellet in 0.1M phosphate-buffered saline (PBS).
DNA extraction of spirochaetal culture was done by using the DNeasy Tissue kit (Qiagen) in the BioRobot EZ1.
DNA was extracted from the spirochaetal culture and subjected to the PCR for the amplification of the flagellin gene, positive samples amplified a product of 750bp.