The extracted Chla was measured with a spectrofluorometer
(Varian Cary Eclipse) using excitation at 430 nm and emission at 670 nm (slit widths 5 nm).
The life time of the fluorescence decay was measured using a LaserStrobe[TM] TM-30 time-resolved spectrofluorometer
provided by Photon Technology International.
2+] mmol/l (on the atomic absorptive spectrofluorometer
of AF-610-A, China); the level of total glucosamine glucans in mkml/l and hyaluronidase activity in mkml/l by spectrofluorometric method (on the spectrofluorometer
F-96, China) using the modified method of P.
The released fluorescent product, 7-amino-4-trifluoromethylcoumarin (AFC), was read using a Flexstation 3 spectrofluorometer
(Molecular Devices, Sunnyvale, CA) at an excitation wavelength of 400 nm and an emission wavelength of 505 nm.
16) with a Spex FluoroLog-3 spectrofluorometer
(JY Horiba) using either 10-mmpathlength polystyrene cuvettes or multiwell plates with a MicroMax plate reader.
(Perkin Elmer, Germany), the intensity of fluorescence emission for the culture containing A.
The relative fluorescence intensity values of ANS-conjugates were measured with a Shimadzu RF-5301 PC spectrofluorometer
(Kyoto, Japan) at an excitation wavelength of 370 nm and an emission wavelength of 470 nm.
Fluorescence was measured at 360/460 nm with a spectrofluorometer
(Biotek reader FLX 800, Austria).
A central instrument facility with sophisticated Instruments such as flow cytometer, DNA sequencer, CD spectrofluorometer
and confocal microscope is available.
Fluorescence of the dye solutions as well as that of the dye-polymer mixture was measured using Jasco FP-6200 Spectrofluorometer
Prior to the first shampoo and after the final shampoo before combing, hair is analyzed using scanning electron microscopy, emission due to tryptophan with a spectrofluorometer
and heat absorption with a differential scanning calorimeter.
The amount of fluorescence emitted was measured using a spectrofluorometer
with an excitation wavelength of 380 nm and an emission wavelength range of 440 nm (21).