For Southern blotting
of T1 generation of transformants, 20 ug of DNA were digested with EcoRI to hybridization with probes.
slightly enhanced the sensitivity of the PCR, but visual inspection of gel-resolved PCR products from feces was nearly equally sufficient (Table and Figure).
1] DNA was screened by PCR and also by Southern blotting
All high molecular weight DNA extracts were susceptible to complete nuclease digestion (data not shown), as demonstrated for tobacco and soybean DNA in Southern blotting
and detection systems.
restriction fragment length polymorphism (RFLPs), short tandem repeats (STS) or variable number tandem repeats (VNTRs) which can be identified by Southern blotting
or the polymerase chain reaction (PCR).
Families of closely related genes are difficult to analyze using standard techniques--because of high sequence similarity, a closely related gene or pseudogene can easily be mistaken for the target gene during Southern blotting
or PCR amplifications.
This, in turn, is only possible when knowledge of the southern blotting
technique, of the overall procedure and of biology are actively integrated.
The most conserved region of our native FCP amino acid sequence was used to design a 100 bp probe for Southern blotting
The resulting putative auxotrophs will be characterized, sequenced, and confirmed by Southern blotting
SB column indicates TCR[Beta] gene rearrangement positivity as determined by Southern blotting
using TCR[Beta] probe; TGGE, clonality as determined by polymerase chain reaction amplification of TCR[Gamma], region; CTCL, cutaneous T-cell lymphoma, including mycosis fungoides; PTCL, peripheral T-cell lymphoma; LGL, large granular cell leukemia; PLL, prolymphocytic leukemia; PTLD, posttransplant lymphoproliferative disorder; CLL/SLL, chronic lymphocytic leukemia/small lymphocytic lymphoma; FL, follicular lymphoma; MCL, mantle cell lymphoma; MALToma, mucosa-associated lymphoid tissue lymphoma; and LCL, large cell lymphoma.
Examples would be the Appleton layer, Bell's palsy, Brewster's law, Brucellosis, Brownian movement, degrees Kelvin, Dewar flask, Graham's law, Higgs particle, Kerr effect, Liston splint, McLaggan diesel engine, McLaurin's theorem, Newland's law, Rankine cycle, Southern blotting
method, and the Stirling engine.
In situ hybridization, immunocytochemistry, confocal immunoflourescent microscopy, and Southern blotting
demonstrated the presence of gamma tubulin, [alpha]-tubulin, EF1[alpha], and several RNAs homologous to Xenopus RNAs implicated in embryonic axis formation or germ cell determination.