Southern blot

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A method developed by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis using a radioactively- or chemically-labeled DNA probe with sequence similarity

Southern blot

a technique for detecting specific DNA sequences following agar gel electrophoresis of a set of DNA restriction enzyme digestion fragments. The fragments after electrophoresis are transferred to a nitrocellulose or nylon membrane by applying the membrane to the gel; the membrane is probed with a radioprobe that hybridizes to one or more of the separated fragments and used to produce an autoradiogram. Named for Edward Southern.
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Southern blot analysis was performed to establish the methylation status of the promoter region, to detect FMs that failed to amplify on PCR analysis and to differentiate females who were homozygous for a normal-sized FMR-1 allele from female PM/FM heterozygotes.
FMR1 Southern blot analysis is used both to characterize samples with numbers of CGG repeats too large to amplify by the PCR and to determine the methylation status of the gene (11).
DNA Isolation and Southern Blot Hybridization Analysis
To determine the location of each 2b locus, PCR products generated from exons 1, 7, and 9 of 2b9 were used to probe the BAC clone Southern blots.
The expected sizes of the amplicons were ascertained by electrophoresis in agarose gels, and the identity of each PCR product was further verified by Southern blot hybridization.
Therefore, the DNA we used in the Southern blots and PCR experiments was molluscan, not algal.
The presence of sequences complementary to the probe DNA is detected using Southern blot hybridization and autoradiography.
The Southern blots thus imply heteromorphism for one or a few large tandem arrays of Lh1, which was confirmed by in situ hybridization.
The Big Shot III hybridization oven is suited to all types of hybridization and incubation applications, including northern blots, southern blots and microarrays.
The fluorescent ms-PCR assay described here requires 5- to 6-fold less patient DNA than Southern blot analysis (1 [micro]g vs 5-6 [micro]g), with a turnaround time from genomic DNA to diagnostic test results of <2 working days compared with a 1-2 week turnaround time for Southern blots.
In addition, PCR analysis is not appropriate for detection of stable integration of genes, especially in Agrobacterium-mediated transformation, and invariably provides less information than restriction analyses and Southern blots.
In Southern blots, a hybridization signal was only detectable in correspondence of the band of chromosomal DNA.

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