Southern Blot test

Southern Blot test

An analytical method traditionally used in DNA analysis. After a sample of DNA fragments is separated by agarose gel electrophoresis, the fragments are transferred to a solid cellulose support by blotting. The gel is placed between a concentrated salt solution and absorbent paper. Capillary action draws the fragments onto the solid support. The support is then treated with radiolabeled DNA probes.
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References in periodicals archive ?
Due to the labor and reagent costs involved, large Southern blot test volumes typically do not occur.
The larger quantity of BNA available eliminates the need to work with radioisotope-labelled probes which are used to identify small quantities of DNA in Southern blot tests, the current DNA identification method.
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