Southern blot hybridization

Southern blot hybridization

Southern blotting Molecular biology A method delineated by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis. See Autoradiography, Base sequence, Complementary sequence, DNA, Electrophoresis, Probe. Cf Northern blot, Western blot.
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To corroborate the position of inserts in the restriction fragments, Southern blot hybridization was fulfilled using pMC505 as probe (data not shown).
As established by Pfister et al (10) and Ozden et al, (4) HPV subtypes 13 and 32 have been identified as the cause for FEH lesions through polymerase chain reaction (PCR) analysis and Southern blot hybridization, respectively.
Southern Hybridization The presence of a begomovirus was further confirmed by Southern blot hybridization using Cotton leaf curl virus as a general probe for begomoviruses [13].
RacPyV does not appear to be integrated into the host genome because both Southern blot hybridization and RCA analyses indicate that full-length genome exists in an episomal, circular state.
Nuclear integration was analyzed by Southern blot hybridization.
9) More recently, molecular techniques such as Southern blot hybridization, polymerase chain reaction, and in situ hybridization demonstrated direct evidence for an association between EBV and Hodgkin lymphoma.
Diagnostic sensitivity of polymerase chain reaction and Southern blot hybridization for the detection of human papillomavirus DNA in biopsy specimens from cervical lesions.
The PCR product, which represents mRNAs enriched in myelin, is incorporated into vector, transformed into bacteria, and colonies that represent individual cDNAs are screened by southern blot hybridization with full-length cDNA probes of the mRNAs for MBP and MOBP.
albicans isolates, obtained from valve trimmings during processing and when the valve was removed from the recipient, were highly similar by DNA fingerprinting using Southern blot hybridization with the DNA probe Ca3 (4,5).
Typically, the TAT for Southern blot hybridization is 10-14 days, making such testing impractical, particularly to diagnose acute leukemias and monitor patients.
In Southern blot hybridization, electrophoresis of the target DNA precedes transfer of the DNA to the filter.

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