Southern blot hybridization
was performed using DIG High Prime DNA Labeling and Detection Kit 2 (Roche Molecular Systems, Pleasanton, CA, USA).
One empty vector control and five Ubi-hpCP:Hc-Pro primary transgenic lines (T0) were analyzed for transgene copy number by Southern blot hybridization
and for the accumulation of siRNAs by Northern blot hybridization.
To corroborate the position of inserts in the restriction fragments, Southern blot hybridization
was fulfilled using pMC505 as probe (data not shown).
It has been linked to HPV subtypes 13 and 32 using PCR analysis and Southern blot hybridization
. Focal epithelial hyperplasia is benign with no risk of malignant transformation, however, evaluating lesions is prudent to rule out more serious conditions.
Southern Hybridization The presence of a begomovirus was further confirmed by Southern blot hybridization
using Cotton leaf curl virus as a general probe for begomoviruses .
RacPyV does not appear to be integrated into the host genome because both Southern blot hybridization
and RCA analyses indicate that full-length genome exists in an episomal, circular state.
Polymerase chain reaction versus Southern blot hybridization
: detection of immunoglobulin heavy-chain gene rearrangements.
Nuclear integration was analyzed by Southern blot hybridization
. Genomic DNA, prepared from eight putative [T.sub.0] transgenic plants, BnA-3, BnA-15, BnA-22, BnA-29, BnA-39, BnA-46, BnA-53, and BnA-69, was digested with HindIII and hybridized to the labeled 1.3-kb fragment of aroA-M1 probe.
The classic method to diagnose a-thalassemias is the laborious and expensive Southern blot hybridization
. Alternative assays include qualitative gap-PCRs (3,6), which depend on the generation of long PCR products, which is not consistently reproducible (3) in the GC-rich sequences of the [alpha]-globin gene cluster.
Diagnostic sensitivity of polymerase chain reaction and Southern blot hybridization
for the detection of human papillomavirus DNA in biopsy specimens from cervical lesions.
The PCR product, which represents mRNAs enriched in myelin, is incorporated into vector, transformed into bacteria, and colonies that represent individual cDNAs are screened by southern blot hybridization
with full-length cDNA probes of the mRNAs for MBP and MOBP.
albicans isolates, obtained from valve trimmings during processing and when the valve was removed from the recipient, were highly similar by DNA fingerprinting using Southern blot hybridization
with the DNA probe Ca3 (4,5).