Therefore, to further confirm successful targeting at the porcine [beta]-casein locus, we carried out southern blot analysis
using porcine [beta]-casein exon 9 and neo probe in the FGF knock-in fibroblast.
Recent improvements in the detection and sizing of large FMR1 expansion mutations by the PCR have reduced the need for Southern blot analysis
in fragile X testing (26-28).
As expected, Southern blot analysis
consistently detects a higher proportion of IGH rearrangements than PCR analysis.
6 2 How can southern blot analysis
be used to distinguish the transgene from the endogenous T-bet gene?
Although Southern blot analysis
combined with the PCR is the current gold standard in FXS diagnostics, published data concerning relationships of cognitive status with the FMR1 activation ratio obtained with Southern blotting are inconsistent, perhaps owing to differences in sample selection.
2 gene into the orchardgrass genome, transgenic plants were analyzed by genomic DNA PCR and Southern blot analysis
For Southern blot analysis
, 7-10 [micro]g of isolated DNA was digested with EcoRI and NruI and separated on an 8-g/L agarose gel containing Tris-acetate-EDTA buffer (40 mmol/L Tris-acetate and 1 mmol/L EDTA).
Southern blot analysis
by using the ampicillin resistance gene of pBluescript KS (+) (Stratagene, La Jolla, CA) as a probe indicated that a resistance gene was carried on the 50-kb plasmid.
Molecular demonstration of EBV nucleic acids includes ISH methods and other methods in which the DNA is first extracted from the tissue prior to hybridization (ie, dot-blot methods, Southern blot analysis
, and PCR).
The published work, titled "High-resolution methylation polymerase chain reaction for fragile X analysis: Evidence for novel FMR1 methylation patterns undetected in Southern blot analyses," describes the accurate and reproducible assessment of the methylation status in 80 clinical samples, including 39 full mutation specimens, with concordance to the reference method of Southern blot analysis
Until now such large rearrangements have been mainly detected by Southern blot analysis
(17, 18), which is time-consuming, highly laborious, and requires large amounts of DNA.
3,4,6,7] Intravascular lymphomatosis was originally believed to be a malignant proliferation of endothelial cells; however, further studies, including immunohistochemistry, Southern blot analysis
, and polymerase chain reaction gene rearrangement, have identified a B-cell lineage in the majority of cases.