Southern blot analysis


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Related to Southern blot analysis: Southern blotting method, Southern transfer, DNA blotting

South·ern blot a·nal·y·sis

(sŭdh'ĕrn),
a procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridized with complementary (labeled) nucleic acid probes.

Southern blot analysis

n.
A technique for identifying specific sequences of DNA in which DNA fragments are separated by electrophoresis, transferred to nitrocellulose, and identified with a suitable probe.

South·ern blot a·nal·y·sis

(sŭdh'ĕrn blot ă-nal'i-sis)
A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridized with complementary (labeled) nucleic acid probes.

Southern,

M.E., 20th century English biologist.
Southern blot analysis - a procedure to separate and identify DNA sequences.
References in periodicals archive ?
(b) The copy numbers of pFJ1-pro3916, pFJ4-pro3916, and pFJ6-pro3916 were determined by Southern blot analysis. Sau3AI-digested total DNA from J7-F cells transformed with pFJ1 (lanes 2-4), pFJ4 (lanes 6-8), or pFJ6 (lanes 10-12) was hybridized with the DIG-labeled 3916 pro sequence.
8.6 2 How can southern blot analysis be used to distinguish the transgene from the endogenous T-bet gene?
In the study, two of five colonies in the FGF knock-in fibroblast were detected by southern blot analysis. Three PCR positive colonies were not detected by southern blot analysis.
Based on the Southern blot analysis and sequence data, it was determined that the bar gene cassette and the GmFad2-1b gene cassette have been inserted into the soybean genome (Figure 4).
All samples were previously characterized by PCR and/or Southern blot analysis (24, 25).
For Southern blot analysis, 944 results were reported, with a successful response rate of 95%.
At the seedling stage, the transgenics were screened by PCR followed by Southern blot analysis for the segregation pattern of the transgenes.
By performing Southern blot analysis, we confirmed the presence of stx1B in the O153:H- and O153:117 isolates, including the strain isolated from the rabbit infected with EPEC.
In this study, we also compared methylation levels of exonic (FREE2 CpG units 1 and 2) and intronic (FREE2 CpG units 6-12) sequences, the FMR1 activation ratios determined with methylation-sensitive Southern blot analysis of the NruI restriction site (within the CpG island), and the results of FMRP immunostaining of blood lymphocytes (28).
Southern blot analysis was achieved using PCR and RTPCR products.
Southern Blot Analysis of the HVA1 Gene in R3 Progeny Plants
Southern blot analysis by using the ampicillin resistance gene of pBluescript KS (+) (Stratagene, La Jolla, CA) as a probe indicated that a resistance gene was carried on the 50-kb plasmid.

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