Southern blot

(redirected from Southern blot analyses)
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Related to Southern blot analyses: Northern blot analysis, RFLP, Western blot analysis
A method developed by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis using a radioactively- or chemically-labeled DNA probe with sequence similarity

Southern blot

a technique for detecting specific DNA sequences following agar gel electrophoresis of a set of DNA restriction enzyme digestion fragments. The fragments after electrophoresis are transferred to a nitrocellulose or nylon membrane by applying the membrane to the gel; the membrane is probed with a radioprobe that hybridizes to one or more of the separated fragments and used to produce an autoradiogram. Named for Edward Southern.
References in periodicals archive ?
This poster describes the evaluation of two sets of AmplideX[TM] FMR1 PCR Reagents for the accurate sizing of a broad range of CGG repeats in the fragile X mental retardation (FMR1) gene on the AB 3130 (RUO* PCR reagents) or the Beckman Vidiera (custom RUO* reagents) capillary electrophoresis instruments, and compares the results with Southern blot analyses.
Segregation was 3:1 for PHV119 and 15:1 for PHV122 (Table 3), results that agree with the Southern blot analyses.
For this, time-consuming Southern blot analyses of the CYP21 locus are still necessary (26, 30).
There was a 100 percent concordance with previously obtained results from PCR and Southern blot analyses.

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