Southern blot


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Related to Southern blot: Northern blot
A method developed by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis using a radioactively- or chemically-labeled DNA probe with sequence similarity

Southern blot

a technique for detecting specific DNA sequences following agar gel electrophoresis of a set of DNA restriction enzyme digestion fragments. The fragments after electrophoresis are transferred to a nitrocellulose or nylon membrane by applying the membrane to the gel; the membrane is probed with a radioprobe that hybridizes to one or more of the separated fragments and used to produce an autoradiogram. Named for Edward Southern.
References in periodicals archive ?
Therefore, to further confirm successful targeting at the porcine [beta]-casein locus, we carried out southern blot analysis using porcine [beta]-casein exon 9 and neo probe in the FGF knock-in fibroblast.
018) with the FMR1 activation ratio determined using methylation-sensitive Southern blot, which represents the methylation status of the FMR1 CpG island 5' of the expansion on normal-size alleles in these females.
The performance of the participating laboratories was very good, by both Southern blot and PCR analysis.
Instead of southern blot analysis, students often propose to use another technique, the polymerase chain reaction (PCR) technique, to determine the copy number.
Correlation of FMR1 Southern Blot Analysis and AmplideX([R]) mPCR for Detection of Size and Methylation Mosaicism in Fragile X Full Mutation Alleles" (abstract G33).
Southern blot hybridizations and RCA were performed to ascertain whether RacPyV existed in episomal, integrated, or a combination of these forms.
What are the steps in the machine, state the temperature Southern blot procedure and and time of annealing.
One key hurdle to the implementation of a population-based carrier screen for FXS is technical: The conventional approach for detecting FMR1 expansions involves a PCR-based assay, which is supplemented by Southern blot analysis because of the high GC content of the FMR1 repeats and the large sizes of disease-causing expansions.
Edwin Southern, inventor of the Southern Blot, published the first description of nucleic acid blotting in the pages of JMB.
The technique, which uses triplet-primed polymerase chain reaction (PCR) with capillary electrophoresis (CE) on specimens of blood, saliva, and dried blood-filter paper spots, can replace the Southern blot technique in 99% of cases without compromising accuracy.
A Southern blot of BamHI-digested genomic DNA was hybridized using an EBV terminal repeat probe XhoI.
False negatives in this study were excluded by subsequent analysis with the Southern blot technique.

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