The mean TRF length by Southern blotting
of normal TIG-3 cells was 8.4 kbp at 36 PDLs, 6.2 kbp at 62 PDLs, 5.9 kbp at 78 PDLs, and 5.8 kbp at 83 PDLs, at which TIG-3 cells senesced.
Genomic DNA was digested under the same conditions as described for Southern blotting
For Southern blotting
of T1 generation of transformants, 20 ug of DNA were digested with EcoRI to hybridization with probes.
As a fast and inexpensive alternative to a live demonstration, I provide an animated slideshow on our school website that has worked well for me as I have walked students through Southern blotting
The presence of the pEGFP-[N.sub.1] DNA was screened by PCR and also by Southern blotting
analysis.The results of different groups are shown in Table 3.
4) went into considerable detail regarding various techniques for gene identification such as electrophoresis, polymerase chain reaction, Southern blotting
, and so forth.
Low variability in yield among sample wells, likely due to the relative thoroughness and evenness of mechanical grinding (Michaels and Amasino, 2001; Hill-Ambroz et al., 2002), makes DNA concentration measurement and adjustment unnecessary for Southern blotting
. Examples of application to difficult plants such as hop, tobacco, and soybean are given, including representative data from a large-scale analyses by Southern blotting
of soybean primary transformants and subsequent sexual segregations of transgenes (Olhoft et al., 2003; Olhoft et al., 2004).
It was then digested with restriction enzymes, separated using gel electrophoresis, and transferred to a nitrocellulose membrane by a Southern blotting
In two families with Hemophilia A and both families with Hemophilia B the causative mutation could be detected by Southern blotting
, polymerase chain reaction or sequence analysis.
a concurrent period of Molecular Genetics identified with PCR, RT-PCR, DNA fingerprinting, micro array analysis, flow cytometry, and Northern and Southern Blotting
. Works on the lipoma in each era are presented and identify the major cytogenetic qualities of the benign lipoma: cytogenetic heterogeneity with over twenty identified karyotypes; nonconcordance with gross morphology in size, shape, depth, location or sex; broad patterns of congruence with histopathological classifications, and limited utility in behavioral predictability.
Families of closely related genes are difficult to analyze using standard techniques--because of high sequence similarity, a closely related gene or pseudogene can easily be mistaken for the target gene during Southern blotting
or PCR amplifications.
This, in turn, is only possible when knowledge of the southern blotting
technique, of the overall procedure and of biology are actively integrated.