Southern blotting


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A method developed by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis using a radioactively- or chemically-labeled DNA probe with sequence similarity
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

Southern blotting

A method of identifying a fragment of DNA containing a specific sequence of bases. A mixture of fragments, produced by cutting DNA with restriction enzymes, is separated on a gel block by ELECTROPHORESIS. The fragments are denatured to single strand DNA and transferred, by blotting, to a nitrocellulose sheet. The position of the desired fragment can then be shown by hybridizing it with a DNA probe labelled with radioactive phosphorous that will cause a black line on an X-ray film. The method was named after its developer Edward M. Southern. A similar technique for RNA analysis has been humorously called ‘Northern blotting’ and the play on words has been extended to include ‘WESTERN BLOTTING’.
Collins Dictionary of Medicine © Robert M. Youngson 2004, 2005

Southern transfer

or

Southern blotting

a method of transferring BANDS of DNA, separated by GEL ELECTROPHORESIS, to a membrane support. The technique is named after E. Southern, who devised it. Compare NORTHERN TRANSFER.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
The mean TRF length by Southern blotting of normal TIG-3 cells was 8.4 kbp at 36 PDLs, 6.2 kbp at 62 PDLs, 5.9 kbp at 78 PDLs, and 5.8 kbp at 83 PDLs, at which TIG-3 cells senesced.
Genomic DNA was digested under the same conditions as described for Southern blotting.
For Southern blotting of T1 generation of transformants, 20 ug of DNA were digested with EcoRI to hybridization with probes.
As a fast and inexpensive alternative to a live demonstration, I provide an animated slideshow on our school website that has worked well for me as I have walked students through Southern blotting (http://biology.williams.edu/faculty-staff/ddean/publications/).
The presence of the pEGFP-[N.sub.1] DNA was screened by PCR and also by Southern blotting analysis.The results of different groups are shown in Table 3.
4) went into considerable detail regarding various techniques for gene identification such as electrophoresis, polymerase chain reaction, Southern blotting, and so forth.
Low variability in yield among sample wells, likely due to the relative thoroughness and evenness of mechanical grinding (Michaels and Amasino, 2001; Hill-Ambroz et al., 2002), makes DNA concentration measurement and adjustment unnecessary for Southern blotting. Examples of application to difficult plants such as hop, tobacco, and soybean are given, including representative data from a large-scale analyses by Southern blotting of soybean primary transformants and subsequent sexual segregations of transgenes (Olhoft et al., 2003; Olhoft et al., 2004).
It was then digested with restriction enzymes, separated using gel electrophoresis, and transferred to a nitrocellulose membrane by a Southern blotting mechanism.
In two families with Hemophilia A and both families with Hemophilia B the causative mutation could be detected by Southern blotting, polymerase chain reaction or sequence analysis.
a concurrent period of Molecular Genetics identified with PCR, RT-PCR, DNA fingerprinting, micro array analysis, flow cytometry, and Northern and Southern Blotting. Works on the lipoma in each era are presented and identify the major cytogenetic qualities of the benign lipoma: cytogenetic heterogeneity with over twenty identified karyotypes; nonconcordance with gross morphology in size, shape, depth, location or sex; broad patterns of congruence with histopathological classifications, and limited utility in behavioral predictability.
Families of closely related genes are difficult to analyze using standard techniques--because of high sequence similarity, a closely related gene or pseudogene can easily be mistaken for the target gene during Southern blotting or PCR amplifications.
This, in turn, is only possible when knowledge of the southern blotting technique, of the overall procedure and of biology are actively integrated.

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