2]) were involved in the relaxing effect of SC, endotheliumintact rings were incubated with L-NAME (a NO synthase inhibitor; 100 [micro]M), MB (a soluble guanylyl cyclase
inhibitor; 10 [micro]M), Rp-cAMP (a cAMP antagonist; 10 [micro]M), or Indomethacin (INDO; a non-selective inhibitor of cyclooxygenase, 10 [micro]M) for 60 min prior to the application of PE, respectively.
Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and soluble guanylyl cyclase
alpha1 and beta1 subunits in the ovary of fetal neonatal and immature pigs.
Additionally, the involvement of the nitric oxide pathway was also investigated by pretreating the mice with a nitric oxide synthase inhibitor, a nitric oxide donor, a soluble guanylyl cyclase
enzyme inhibitor or an ATP-sensitive K+channel blocker 15 minutes prior to rhodiola administration.
Sub-second kinetics of the nitric oxide receptor, soluble guanylyl cyclase
, in intact cerebellar cells.
2 The precise mechanism of action is not yet clear, but it appears that HU increases nitric oxide levels, causing soluble guanylyl cyclase
activation with a resultant rise in cyclic GMP, and the activation of gamma-globin synthesis necessary for HbF.
Binding of NO to the heme moiety of soluble guanylyl cyclase
activates the guanylyl cyclase (GC), which increases the concentration of cyclic Guanosine 3'-5' monophosphate (cGMP).
In general, NO is thought to interact with the iron-containing enzymes, such as soluble guanylyl cyclase
(sGC) (Ignarro et al.
1 NO stimulates soluble guanylyl cyclase
(sgc) to produce cGMP in endothelial cells.
Since ODQ interferes directly with soluble guanylyl cyclase
activated by NO (22), relaxation induced by ayanin may be related to the production of NO, resulting in an increase of the cGMP in the endothelium.
cGMP mediates the vascular and platelet actions of nitric oxide: confirmation using an inhibitor of the soluble guanylyl cyclase
To establish the pharmacological concentrations necessary to stimulate cytosolic cGMP production in freshwater shrimp chromatophores, we generated dose-response curves for the soluble guanylyl cyclase
activators sodium nitroprusside (SNP) and morpholinosydnonimine (SIN-1), which are nitric oxide donors (Fig.
The proangiogenic activity of NO is mediated by activation of soluble guanylyl cyclase