2]) were involved in the relaxing effect of SC, endotheliumintact rings were incubated with L-NAME (a NO synthase inhibitor; 100 [micro]M), MB (a soluble guanylyl cyclase inhibitor; 10 [micro]M), Rp-cAMP (a cAMP antagonist; 10 [micro]M), or Indomethacin (INDO; a non-selective inhibitor of cyclooxygenase, 10 [micro]M) for 60 min prior to the application of PE, respectively.
The endothelium-dependent relaxation was abolished by MB, indicating that soluble guanylyl cyclase is target site for SC.
Additionally, the involvement of the nitric oxide pathway was also investigated by pretreating the mice with a nitric oxide synthase inhibitor, a nitric oxide donor, a soluble guanylyl cyclase
enzyme inhibitor or an ATP-sensitive K+channel blocker 15 minutes prior to rhodiola administration.
cGMP mediates the vascular and platelet actions of nitric oxide: confirmation using an inhibitor of the soluble guanylyl cyclase
The proangiogenic activity of NO is mediated by activation of soluble guanylyl cyclase
The paper elucidates the means by which nitric oxide and carbon monoxide bind to a protein called soluble guanylyl cyclase
(sGC) which is critical in the formation of another protein, cGMP, which induces the relaxation of blood vessels.
In current investigation, it was observed that relaxant effect produced by 1 was drastically inhibited when tested in the presence of NAME (10 [mu]M, a nitric oxide synthase inhibitor), ODQ (1 [mu]M, a soluble guanylyl cyclase
inhibitor), and atropine (1 [mu]M, an antagonist of muscarinic receptor) (Figs.
To verify further the involvement of nitric oxide/cyclic guanosine monophosphate signaling pathway, the i-NAME (an inhibitor of NO synthase) 100 [micro]M or MB (a soluble guanylyl cyclase inhibitor) 10.
Diffusible nitric oxide gas penetrates vascular smooth muscle and activates soluble guanylyl cyclase which catalyzes which catalyzes guanosine triphosphate to form cyclic guanosine monophosphate (Da Silva Rde et al.
To verify further the involvement of nitric oxide/cyclic guanosine monophosphate signaling pathway, the L-NAME (an inhibitor of NO synthase) 100 [micro]M or MB (a soluble guanylyl cyclase
The role of nitric oxide (NO) was confirmed by an increase in colonic NO production after schisandrin incubation, and the inhibition on the schisandrin responses by soluble guanylyl cyclase inhibitor 1H-[1,2,4] oxadiazolo[4,3-cd-quinoxalin1-one (1-30 p.
A soluble guanylyl cyclase (GC) inhibitor ODQ was used to test the involvement of GC-dependent pathways in the relaxant effect of schisandrin.