Integrons are mobile elements that participate in a powerful site-specific recombination
system to capture, accumulate, excise, and organize gene cassettes (11,31-33).
In both prokaryotic and eukaryotic organisms, various site-specific recombination
reactions are conserved, and these systems generally involve specific enzymes and unique asymmetric DNA sequences [8-10].
These pleiotrophic effects include deregulation of type 1 site-specific recombination
. It has been indicated recently that H-NS protein binds to upstream position of fimA promoter and accelerates its activity in ON orientation switch.14,15 The binding region of H-NS protein located immediately downstream of fimE, encompasses site I binding of IHF and extending into the switch region.
In dam-suppressor mutants, Chr1 and Chr2, fusions had occurred either by homologous recombination between IS elements or site-specific recombination
Genetically, the differences occurred in tcpA, which encodes the major adherence antigen rstR that regulates site-specific recombination
of CTX9 phage and ctxB that encodes the B subunit of cholera toxin.
Gateway(r) Technology, developed by Invitrogen, is a universal cloning method which uses the site-specific recombination
properties of bacteriophage lambda (Landy, 1989).
Xer Site-Specific Recombination
: Promoting Chromosome Segregation.
Taking the dream one step closer to industrial reality, the team has developed data proof-read/correction and random access modules, in addition to an encryption module, all using site-specific recombination
of the inversion type, specifically, R64 Shufflon-Specific Recombinase, a type of Rci-mediated recombination.
(Johnston, IA) has patented methods of controlling gene silencing using site-specific recombination
. A variety of constructs are provided which are useful for conditional or regulated gene silencing in plants, comprising a suite of constitutive, inducible, tissue-specific or developmental stage-specific promoters operably linked to target sequences (TS).
One of the most efficient procedures for generating single-copy plants is site-specific integration of foreign genes mediated by heterologous site-specific recombination
systems (Albert et al., 1995; Srivastava et al., 2004).
Remaining chapters discuss the use of homologous recombination and site-specific recombination
to disrupt or modify endogenous genes and genomes; strategies for controlling transgene expression; position effects, dosage effects, genetic background, transgene structure, and other aspects of transgene behavior that can generate unexpected results in gene transfer experiments; the use of gene transfer to achieve the targeted or random inactivation of endogenous genes; and the major application of gene transfer, including commercial synthesis of recombinant proteins, domestic animal improvement, disease modeling, and gene therapy.
Gateway Cloning Technology is based on the well-characterized lambda phage site-specific recombination