site-directed mutagenesis

(redirected from Site-specific mutagenesis)

site-·di·rec·ted mu·ta·gen·e·sis

the controlled alterations of selected regions of a DNA molecule.

site-directed mutagenesis

An experimental technique in which a mutation is inserted in DNA at a particular site to determine the site’s function, if any. In site-directed mutagenesis, nucleotides are altered, resulting in substitution of amino acids in a protein of known function, which serves to identify the DNA sequence’s role (e.g., as a receptor or ion channel).
References in periodicals archive ?
Also, the general principles of the molecular dynamics approach and its applications in the study of conformational changes of proteins due, for example, to site-specific mutagenesis and drug/contaminant-protein interactions were explained.
Rapid and efficient site-specific mutagenesis without phenotypic selection.
Genoplasty is a unique site-specific mutagenesis technology that allows precise changes to be inserted into target genes which may be used to introduce genetic improvements without the need for foreign DNA.
Site-specific mutagenesis played an important role in the solution of the structure in that the cysteine mutants C865, C1 14S, and C173S were used to help locate the positions of heavy atoms and to align the sequence with the model derived from the experimental phases.
Mutagenic potential of [O.sup.4]-methylthymine in vivo determined by an enzymatic approach to site-specific mutagenesis. Proc.
Site-specific mutagenesis by a propanodeoxyguanosine adduct carried on an M13 genome.
His group has also probed the factors determining and controlling the catalytic activities and specificities of the enzymes of asymmetric synthetic value, using molecular graphics, molecular dynamics, and site-specific mutagenesis approaches.
For about a decade, researchers have used a technique called site-specific mutagenesis to interchange amino acids at individual locations in a protein and study the functional consequences.

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