Michael Smith "for his fundamental contributions to the establishment of oligonucleotide-based, site-directed mutagenesis
and its development for protein studies"
Libraries built on the BioXp System support a variety of downstream applications including gene engineering, gene modification, and site-directed mutagenesis
of porcine Nanog promoter sequences
In order to analyze the most common PTGS2 haplotypes, different PR and 3'-UTR variants were generated by site-directed mutagenesis
using overlap extension PCR (14).
Using site-directed mutagenesis
and the CRISPR-associated protein 9 (CRISPR/Cas9) system, they engineered DNA fragments carrying a variety of clinically important alterations, including the EGFR T790M, L858R and exon 19 deletion, KRAS G12D mutation, and more complex EML4-ALK rearrangements and mixed them with micrococcal nuclease-digested HEK293 cell-line DNA to obtain DNA samples with varying allelic fractions of mutant DNA.
In general, this has the advantage of being inexpensive and technically easy, since site-directed mutagenesis
techniques are well-developed.
British-born Canadian biochemist Michael Smith, HFCIC, shared the 1993 Nobel Prize in Chemistry with American Kary Mullis for their work developing "site-directed mutagenesis
," which allows researchers to introduce specific, engineered mutations into a DNA sequence.
of different genes can provide important information about their functions.
Constructing Infectious HCV with Site-Directed Mutagenesis
and Verifying Its Drug-Resistance.
Coupland, "Site-directed mutagenesis
in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles," Planta, vol.
The mutant  putative binding site was generated using QuikChange site-directed mutagenesis
kit (Figure 3(a)).