silver stain

(redirected from Silver staining)

sil·ver stain

any of a variety of stains (for example, Bielschowsky, Gomori silver, impregnation stains) that employ alkaline silver nitrate solutions to stain connective tissue fibers (reticulin, collagen), calcium salt deposits, spirochetes, neurologic tissue, and nucleolar organizer regions.
A metallic silver-based stain which is used to highlight basement membranes, and is of greatest use in glomerulonephritides, many of which have thickening or other glomerular basement membrane changes

silver

1. a chemical element, atomic number 47, atomic weight 107.870, symbol Ag. See Table 6. It is used in medicine for its caustic, astringent and antiseptic effects. Experimental poisoning with silver salts causes myopathy.
2. a coat color in dogs, foxes.

silver amalgam
see amalgam.
silver collie syndrome
see canine cyclic hematopoiesis.
silver grass
aristidacontorta.
silver halide
any of the silver salts with halogens including bromine, chlorine, iodine used in photographic emulsion.
silver iodide
soluble silver salt used in cloud seeding but presents no toxicological risk to local grazing cattle.
silver-leaf ironbark
eucalyptusmelanophloia.
silver-leafed nightshade
solanumelaeagnifolium.
silver nitrate
colorless or white crystals, used as a caustic and local anti-infective.
silver nitrate (toughened)
a mixture of silver nitrate with hydrochloric acid, sodium chloride or potassium nitrate, occurring as white crystalline masses molded into pencils or cones, called caustic pencils; a convenient means of applying silver nitrate locally. Called also lunar caustic.
silver protein
silver made colloidal by the presence of, or combination with, protein; an active germicide with a local irritant and astringent effect.
silver stain
a method of demonstrating flagella on bacteria, or for visualizing very thin bacteria, such as leptospires.
silver sulfadiazine
the silver salt of sulfadiazine, having bactericidal activity against many gram-positive and gram-negative organisms, as well as being effective against yeasts; used as a topical anti-infective for the prevention and treatment of wound sepsis in patients with second and third degree burns.
silver weed
References in periodicals archive ?
Silver staining showed thickened glomerular basement membranes with small projections (spikes).
Glycoform-specific LPSs are difficult to recover from silver-stained PAGE gels, because the silver staining process chemically modifies and irreversibly fixates LPSs into the gel.
The SSCP bands were visualized by silver staining following the protocol of Bassam et al.
The silver staining technique has been used to locate ion transporting organs in a number of aquatic insects and crustaceans (Krogh 1939; Ewer & Hattingh 1952; Copeland 1967; Jarial et al.
1,2] Warthin-Starry silver staining is the gold standard for diagnosis, revealing clusters of bacilli.
Samples were analyzed for the presence of rotavirus by polyacrylamide gel electrophoresis (PAGE) followed by silver staining (HERRING, 1982), and samples were analyzed for the presence of Giardia sp.
The researchers used several methods for examining the cultures for spirochetes including light and darkfield microscopy, silver staining, immunohistochemical staining, molecular hybridization, and PCR analysis.
Discard ethanol, add 200 mL double distilled water and shake for 10 min at room temperature; (3) Sensitization: remove water, then add 100 mL silver staining sensitizing solution (1x), shake for 2 min at room temperature at a shaking speed of 60-70 rpm (revolutions per minute); (4) Silver staining: add 100 mL silver solution (1x), shake for 10 min at a shaking speed of 60-70 rpm, and washed by double distilled water for 1min; (5) Color: discard water, add 100 mL silver staining solution, shake for 3-10 min until the ideal protein band appears; (6) Termination: remove from silver staining solution, add 100 mL silver staining terminated liquid (1x), shake for 10 min at room temperature, and washed by double distilled water for 2 min.
12) Silver staining techniques are not specific for Leptospira and will also stain other spirochaetes and various other bacteria.
5) In cases with cutaneous involvement, skin biopsy with histological hematoxylin and eosin (H&E) and silver staining may reveal the organism, although the skin biopsy may not be diagnostic (i.
The technique of Sumner (1972) was used for C-banding of sample preparations whereas the technique of Howell and Black (1980) was used for silver staining.
The acrylamide gels were stained by means of the silver staining protocol described by Creste et al.