In 2008, 2 independent groups demonstrated that NIPT of trisomy 21 (T21) could be achieved using massively parallel shotgun sequencing (13, 14), ushering in a new era of NIPT and opening new possibilities for the use of these technologies in clinical practice.
Craig Venter institute, lead to the first genome of a bacterium, (4,5) marking the first use of the shotgun sequencing approach that Venter and the Human Genome project would use to map the human genome.
Genome assembly refers to the process of taking a large number of short DNA sequences all of which generated by a shotgun sequencing project and putting them back together to create a representation of the original chromosomes from which the DNA originated .
2013) used shotgun sequencing to compare microbial metabolic potential between two sites on the French Mediterranean coast, one with heavy arsenic pollution and another with arsenic concentrations well below toxic levels.
The involvement of the microbiome in autoimmune diseases has been proven, and the technological transition to the second generation community-wide full-genome shotgun sequencing is potentially enabling deeper, mechanistic, and functional microbiome profiles.