Shotgun Sequencing


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The sequencing of a large DNA segment by sequencing of randomly-derived subsegments, whose order and orientation within the large segment are unknown until the final assembly of overlapping sequences.
References in periodicals archive ?
However, Creative Biolabs just took a massive leap forward into the new era of this field, with unchallenged ability to achieve 100% accuracy in antibody sequencing by precisely distinguishing between leucine and isoleucine, based on the proprietary Database Assisted Shotgun Sequencing (DASS) technology.
We performed whole-genome shotgun sequencing and assembly as described previously (10) (Appendix).
Several molecular biology techniques were used to assess the characterization of the gut microbiome: denaturing gradient gel electrophoresis (DGGE), fluorescence in situ hybridization probes (FISH), metagenomics shotgun sequencing, and characterization of the 16S rRNA genes in a sample, and groups specific real-time polymerase chain reaction (PCR) are some of the assays.
More recently, the advent of next-generation sequencing platforms has allowed unbiased approaches to high-resolution characterization of complex microbial communities known as metagenomic shotgun sequencing (MSS).
Shotgun sequencing of Yersinia enterocolitica strain W22703 (biotype 2, serotype O:9): genomic evidence for oscillation between invertebrates and mammals.
RNA-Seq, also called whole transcriptome shotgun sequencing, uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given time, which is a more sensitive and accurate way to investigate genome-wide gene expression than microarray.
Whole-genome shotgun sequencing revealed one type of bacteria, Lactobacillus reuteri, which was reduced more than nine-fold in the microbiome of mice born to mothers on the high-fat diet.
Best known for being among the first to map the human genome using "shotgun sequencing" of human DNA, Venter heads up a non-profit genomic research institute named after him.
Craig Venter institute, lead to the first genome of a bacterium, (4,5) marking the first use of the shotgun sequencing approach that Venter and the Human Genome project would use to map the human genome.