Shotgun Sequencing

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The sequencing of a large DNA segment by sequencing of randomly-derived subsegments, whose order and orientation within the large segment are unknown until the final assembly of overlapping sequences.
References in periodicals archive ?
We extracted plasmid DNA from 9 isolates encoding NDM-1, 11 isolates encoding KPC, and 3 isolates encoding CTX-M-15 and performed shotgun sequencing on those plasmid preparations.
QNatal Advanced is a lab-developed test developed and validated by Quest Diagnostics using massively parallel shotgun sequencing (MPSS), a type of next-generation sequencing technology, with GC correction.
The original NIPT methodology is known as massively parallel shotgun sequencing (MPSS).
Human papillomavirus community in healthy persons, defined by metagenomics analysis of human microbiome project shotgun sequencing data sets.
Nextgen sequencing technologies have enabled noninvasive prenatal testing of fetal aneuploidies by shotgun sequencing of maternal plasma DNA during pregnancy (1).
The involvement of the microbiome in autoimmune diseases has been proven, and the technological transition to the second generation community-wide full-genome shotgun sequencing is potentially enabling deeper, mechanistic, and functional microbiome profiles.
In this study, we analyzed the complete genome of goat rumen microbes obtained using a shotgun sequencing method.
The work to complete the shotgun sequencing of the wheat genome will help to improve programs on breeding and adaptation in Asia and Sub-Saharan Africa for wheat crops that could be drought tolerant and resistant to weeds, pests and diseases.
Ulrich Certa, Global Head Molecular Toxicology, and his team first applied a shotgun sequencing strategy using the Genome Sequencer FLX System from Roche's 454 Life Sciences division in combination with other next generation sequencing technologies to decode the entire genome of a Macaca fascicularis female of Mauritian origin with 6-fold coverage.
The key was that Venter utilized the most powerful computers to make shotgun sequencing successful.
Mate pairs are two reads from the same clone that are used to assemble a genome in shotgun sequencing.
The genome of Aspergillus oryzae was determined by the whole-genome shotgun sequencing method and verified by the optical mapping method.