Shotgun Sequencing

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The sequencing of a large DNA segment by sequencing of randomly-derived subsegments, whose order and orientation within the large segment are unknown until the final assembly of overlapping sequences.
References in periodicals archive ?
Shotgun sequencing of Yersinia enterocolitica strain W22703 (biotype 2, serotype O:9): genomic evidence for oscillation between invertebrates and mammals.
Whole-genome shotgun sequencing revealed one type of bacteria, Lactobacillus reuteri, which was reduced more than nine-fold in the microbiome of mice born to mothers on the high-fat diet.
In 2008, 2 independent groups demonstrated that NIPT of trisomy 21 (T21) could be achieved using massively parallel shotgun sequencing (13, 14), ushering in a new era of NIPT and opening new possibilities for the use of these technologies in clinical practice.
Craig Venter institute, lead to the first genome of a bacterium, (4,5) marking the first use of the shotgun sequencing approach that Venter and the Human Genome project would use to map the human genome.
These tools fall into two broad categories: polymerase chain reaction (PCR)--based targeted approaches and shotgun sequencing approaches (figure 2), which we explain in detail in the sidebar.
We extracted plasmid DNA from 9 isolates encoding NDM-1, 11 isolates encoding KPC, and 3 isolates encoding CTX-M-15 and performed shotgun sequencing on those plasmid preparations.
The original NIPT methodology is known as massively parallel shotgun sequencing (MPSS).
Genome assembly refers to the process of taking a large number of short DNA sequences all of which generated by a shotgun sequencing project and putting them back together to create a representation of the original chromosomes from which the DNA originated [27].
Human papillomavirus community in healthy persons, defined by metagenomics analysis of human microbiome project shotgun sequencing data sets.
2013) used shotgun sequencing to compare microbial metabolic potential between two sites on the French Mediterranean coast, one with heavy arsenic pollution and another with arsenic concentrations well below toxic levels.
Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing," BMC Microbiology, vol.
The involvement of the microbiome in autoimmune diseases has been proven, and the technological transition to the second generation community-wide full-genome shotgun sequencing is potentially enabling deeper, mechanistic, and functional microbiome profiles.