Shotgun Sequencing


Also found in: Wikipedia.
The sequencing of a large DNA segment by sequencing of randomly-derived subsegments, whose order and orientation within the large segment are unknown until the final assembly of overlapping sequences.
References in periodicals archive ?
However, Creative Biolabs just took a massive leap forward into the new era of this field, with unchallenged ability to achieve 100% accuracy in antibody sequencing by precisely distinguishing between leucine and isoleucine, based on the proprietary Database Assisted Shotgun Sequencing (DASS) technology.
We performed whole-genome shotgun sequencing and assembly as described previously (10) (Appendix).
This works best with sequencing methods, which can sequence very long DNA strands and still have a sufficiently high coverage rate to be able to completely sequence also human genomes.Approaches are suitable for this, which enable sequencing on UFZ already existing high-throughput sequencing systems according to the shotgun sequencing principle by segmentation and linking of long DNA sequences.
Several molecular biology techniques were used to assess the characterization of the gut microbiome: denaturing gradient gel electrophoresis (DGGE), fluorescence in situ hybridization probes (FISH), metagenomics shotgun sequencing, and characterization of the 16S rRNA genes in a sample, and groups specific real-time polymerase chain reaction (PCR) are some of the assays.
More recently, the advent of next-generation sequencing platforms has allowed unbiased approaches to high-resolution characterization of complex microbial communities known as metagenomic shotgun sequencing (MSS).
Shotgun sequencing of Yersinia enterocolitica strain W22703 (biotype 2, serotype O:9): genomic evidence for oscillation between invertebrates and mammals.
RNA-Seq, also called whole transcriptome shotgun sequencing, uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given time, which is a more sensitive and accurate way to investigate genome-wide gene expression than microarray.
Whole-genome shotgun sequencing revealed one type of bacteria, Lactobacillus reuteri, which was reduced more than nine-fold in the microbiome of mice born to mothers on the high-fat diet.
Best known for being among the first to map the human genome using "shotgun sequencing" of human DNA, Venter heads up a non-profit genomic research institute named after him.
Craig Venter institute, lead to the first genome of a bacterium, (4,5) marking the first use of the shotgun sequencing approach that Venter and the Human Genome project would use to map the human genome.