However, Creative Biolabs just took a massive leap forward into the new era of this field, with unchallenged ability to achieve 100% accuracy in antibody sequencing by precisely distinguishing between leucine and isoleucine, based on the proprietary Database Assisted Shotgun Sequencing
We performed whole-genome shotgun sequencing
and assembly as described previously (10) (Appendix).
Several molecular biology techniques were used to assess the characterization of the gut microbiome: denaturing gradient gel electrophoresis (DGGE), fluorescence in situ hybridization probes (FISH), metagenomics shotgun sequencing
, and characterization of the 16S rRNA genes in a sample, and groups specific real-time polymerase chain reaction (PCR) are some of the assays.
More recently, the advent of next-generation sequencing platforms has allowed unbiased approaches to high-resolution characterization of complex microbial communities known as metagenomic shotgun sequencing
of Yersinia enterocolitica strain W22703 (biotype 2, serotype O:9): genomic evidence for oscillation between invertebrates and mammals.
By shotgun sequencing
of human plasma, Dinakaran et al.
RNA-Seq, also called whole transcriptome shotgun sequencing
, uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given time, which is a more sensitive and accurate way to investigate genome-wide gene expression than microarray.
Whole-genome shotgun sequencing
revealed one type of bacteria, Lactobacillus reuteri, which was reduced more than nine-fold in the microbiome of mice born to mothers on the high-fat diet.
Best known for being among the first to map the human genome using "shotgun sequencing
" of human DNA, Venter heads up a non-profit genomic research institute named after him.
"Metagenomics relies on shotgun sequencing
," explained Kaufman.
Craig Venter institute, lead to the first genome of a bacterium, (4,5) marking the first use of the shotgun sequencing
approach that Venter and the Human Genome project would use to map the human genome.