Shine-Dalgarno sequence


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Shine-Dal·gar·no se·quence

(shīn dăl'gahr-nō),
a purine-rich, untranslated region of mRNA upstream from the initiation codon in prokaryotes; assists in aligning the mRNA on the ribosome.
[J. Shine, L. Dalgarno]

Shine-Dalgarno (SD) sequence

a PURINE-rich sequence (5′-AGGAGGU-3′) about six to eight bases UPSTREAM of the start CODON for TRANSLATION, on bacterial mRNA. The sequence is complementary to, and base pairs (see BASE PAIRING with, a sequence near the 3′-end of the 16S ribosomal RNA of the RIBOSOME. It acts as a RIBOSOME BINDING SITE and is important in ribosome alignment for efficient translation. The sequence is named after J Shine and 1 Dalgarno, who discovered it in ESCHERICHIA COLI .

Shine-Dalgarno sequence

part of the leader sequence preceding the start codon of mRNA that is AGGAGGU or a variant thereof that binds the mRNA to a complementary sequence in the 16S component of rRNA so that translation of the mRNA can proceed.
References in periodicals archive ?
CTTGAC and TTATC sequences located at positions 80 and 113 nt, respectively, upstream from the Shine-Dalgarno sequence corresponded to the putative utative ns 80 and 113ion of a single A putative catalytic domain and a cellulose-binding domain were found between 872 and 1,851 and between 1,920 and 2,252, respectively, the domains were connected by a Ser- and a Thr-rich linker region between 1,852 and 1,919.
Key words: translation, untranslated leader, Shine-Dalgarno sequence, leaderless mRNA
The Shine-Dalgarno sequence [2] is a consensus (5'-AAGGAGGU-3') initially characterized in mRNAs of Escherichia coli.
Correlations between Shine-Dalgarno sequences and gene features such as predicted expression levels and operon structures.