A microscopic examination of the bone marrow specimen revealed no Sezary cell
Thus, loss of CD7 expression on the surface of Sezary cells
and alteration of their glycosylation (characterized by the occurrence of sialylated core 1 O-glycans) most probably contribute to the resistance of these cells to a variety of apoptosis-inducing agents, including Gal 1, and to the poor prognosis of T cell lymphoma (54).
A peripheral blood smear reveals Sezary cells
, which are large, hyperconvoluted lymphocytes.
Mild nuclear-contour irregularities may occur, but the classic cerebriform nuclei of Sezary cells
are not present.
Meyerson, "Diminished CD3 expression is useful for detecting and enumerating Sezary cells
," American Journal of Clinical Pathology, vol.
The various types of malignancy associated with PEO are T and B cell lymphoma, leukemia, adenocarcinoma of gut and cutaneous T cell lymphoma.1,4,5 There are reports of PEO associated with AIDS and drug ingestion.6,7 The association of PEO with malignancy and laboratory features like circulating Sezary cells
, hypereosinophilia and raised IgE levels point towards the heterogenous nature of this entity.8 When associated with lymphoma, the dermatosis may appear several years before the diagnosis of malignancy or vice versa, so the association of two pathologies in a possible nosological overview has been stressed.9
One reason for which ECP may be ineffective in patients with patch stage MF is the fact that the majority of patients treated had low levels of circulating Sezary cells
in the peripheral blood (26).
Other important laboratory studies include complete blood count--with differential--as well as peripheral smear looking for Sezary cells
, lactic dehydrogenase (LDH), liver function tests, and uric acid.
Here she was responsible for QC/QA and staff training as well as performing bone marrow diffs, smears, and initial reading for Sezary cells
and diagnostic differentials on the full spectrum of leukemia through to hemoglobinopathies.
Immunophenotypic identification of Sezary cells
in peripheral blood.
Peripheral blood often shows medium-sized Sezary cells
, with convoluted cerebriform nuclei, similar to those seen in tissues.
Bone marrow aspirate showed 10% lymphocyte, 5% plasmo-cytes without Sezary cells
. T-cell clonality studies were performed in the peripheral blood and revealed a genetic rearrangement pat-tern of the beta chain T-cell receptor (TCR-beta) and abnormal T clone in seven metaphases with loss of Y chromosome.