serum-free media


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serum-free media

A well-defined and controlled culture media that has been processed in some form (e.g., filtered to remove inhibitors and/or growth factors). Serum-free media are not necessarily protein-free or chemically defined; most are optimised for the growth requirements of cell-specific applications.
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In the TB exclusion test, 10 [micro]L of TB was added to 10 [micro]L of RPMI-1640 serum-free media containing 10,000 promastigotes, and the viability was immediately determined at 0, 2, 3, 4, 5, and 72 h (23).
Serum-free cell culture: The serum-free media interactive online database.
(a) Recombinant IL6 was added to serum-free media placed in the bottom of a Transwell chamber.
In contrast with fibroblasts, ECs failed to survive beyond three days in any of the serum-free media (Figure 5(a)).
The upper chamber was filled with MDA-MB-231 (5 x [10.sup.4] cells/100 [micro]l) or MCF-7 (1 x [10.sup.5] cells/100 [micro]l) cell suspension and serum-free media containing different concentrations of liensinine or nuciferine.
The media was replaced the next day with serum-free media and/or GSK-3[beta] inhibitor (50, 200, and 1000 nM) and cells were incubated for 60 h.
Before experiments, CHO-pGHR cells were cultured in serum-free media containing 1% BSA for 12 h.
The global cell culture market segmentation is based on product types (equipment, biosafety cabinets, cryostorage equipment, culture systems, incubators, pipetting instruments, roller bottle equipment), and consumables (media, chemically-defined media, classical media, lysogeny broth, protein-free media, serum-free media, specialty media, sera, fetal bovine serum, reagents, albumin, amino acids, attachment factors, growth factors and cytokines, protease inhibitors, thrombin).
Culture of viable human blastocysts in defined sequential serum-free media. Hum.
HBMECs (3 x [10.sup.4] cells/well) containing different concentrations of astaxanthin (3, 10 and 30 [micro]M), and RASMCs (2 x [10.sup.4] cells/well) containing different concentrations of astaxanthin (1, 3 and 10 [micro]M) were seeded in 24-well plates in serum-free media previously coated with growth factor-reduced matrigel matrix (BD Bioscience, San Jose, CA, USA).
"We have developed this new medium using the most up-to-date knowledge of T cell biology to deliver improved performance over serum-containing and other existing commercial xeno-free, serum-free media in supporting ex vivo quality expansion of various human T cell subsets."
The serum-free media contained 7.5 mM glucose, 5 [micro]g/mL insulin, 16.1 [micro]g/mL putrescine, 10% bovine serum albumin, 3.7mg/mL NaHC[O.sub.3], 5.2 mg/L [Na.sub.2]Se[O.sub.3], and 3.6 mg/mL HEPES in minimum essential medium as described [21, 23, 24].
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