Sephadex


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Sephadex

/Seph·a·dex/ (sef´ah-deks) trademark for cross-linked dextran beads. Various forms are used in chromatography.
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The samples after heat treatment was applied on pre equilibrated DEAE sephadex A-50 column using 50 mM sodium acetate buffer pH 6.
On the basis of the previous studies, our research team further used the extraction technique to roughly extract Dahuang first; then isolated and purified it by repeated silica gel column chromatography, sephadex column chromatography, ODS C18 reversed phase silica gel column chromatography and MCI column chromatography; and finally identified it by [sup]1 H-NMR,[sup]13 C-NMR, and DEPT nuclear magnetic resonance spectroscopy.
Gel Filtration: The enzyme thus obtained was further purified by using sephadex G-50 (50x2 cm).
The n-hexane fraction (130 g) was chromatographed on a silica gel column using a petroleum ether : ethyl acetate gradient elution, then purified on a Sephadex LH20 column using methylene chloride for elution, yielding compound 1 (3.
In the present study, chitinase, a PR protein, from peanut seeds was purified to homogeneity by ammonium sulfate precipitation, gel filtration chromatography on Sephadex G-75 column and ion exchange chromatography on Q-Sepharose column.
5 pH) and was passed through a column with Sephadex (pD-10 desalting columns contain Sephadex G-25).
2]), fetuin lyophilized powder, suitable for cell culture (F2379) and Sephadex G-75 were purchased from Sigma-Aldrich (St.
Limited Tenders are invited for Sephadex, Tris Base, Tris Hcl
Entre las tecnicas utilizadas para aumentar la calidad seminal, se encuentran la separacion del plasma seminal (Aurich, 2008), los metodos 'swim-up' o 'swim-down'; la filtracion con lana de vidrio o con Sephadex (Neild, 2013) y la centrifugacion con gradientes de densidad (DGC) o de una capa (SLC), las cuales aumentan la supervivencia espermatica (Morrell et al.