Results obtained are interpreted by the
Scatchard plot (Figure 5 and 6).
Curve [c.sub.Thulium(III)-arsenazo III] NaCl (mol/L) /[c.sub.DNA] a 0.00 0.00 0.05 b 0.20 0.00 0.05 c 0.40 0.00 0.05 d 0.60 0.00 0.05 Curve
Scatchard plot values a r/c = 1.99 x [10.sup.4] - 1.05 x [10.sup.7]r r/c = 4.23 x [10.sup.4] - 1.28 x [10.sup.7]r b r/c = 2.35 x [10.sup.3] - 8.63 x [10.sup.6]r r/c = 1.24 x [10.sup.4] - 8.58 x [10.sup.6]r c r/c = 2.61 x [10.sup.4] - 6.01 x [10.sup.6]r r/c = 9.14 x [10.sup.3] - 9.93 x [10.sup.6]r d r/c = 2.85 x [10.sup.4] - 5.62 x [10.sup.6]r r/c = 1.74 x [10.sup.4] - 1.04 x [10.sup.7]r Curve K (L/mol) n a 1.05 x [10.sup.7] 0.00169 1.28 x [10.sup.7] 0.00207 b 8.63 x [10.sup.6] 0.00027 8.58 x [10.sup.6] 0.00122 c 6.01 x [10.sup.6] 0.00404 9.93 x [10.sup.6] 0.00082 d 5.62 x [10.sup.6] 0.00420 1.04 x [110.sup.7] 0.00149
If these sites are equivalent and independent, relevant experimental data may be analysed toward the thermodynamic binding constant and the number of binding sites in terms of a linear
Scatchard plot [19].
The data were evaluated in the form of a
Scatchard plot, to determine the affinity and binding capacity of the imprinted polymer, expressed by affinity constants (K) and apparent maximum number ([B.sub.max]) of the imprinted polymer sites.
The values of 1/[K.sup.app.sub.d] and [F.sub.max] can be obtained from the slope and the x-intercept of the
Scatchard plot, respectively.
The resulting
Scatchard plot indicated that albumin had 1 high-affinity site and perhaps 2 or more weaker sites (2).
I: The 'logit-log' method and
Scatchard plot. Washington: National Technical Information Service, U.S.
The unchanged structure-activity relations of peptide binding suggest that the detergent did not adversely affect the ligand binding site, but the curvilinear
Scatchard plot does indicate some heterogeneity.
[alpha]] of 5 X [10.sup.9] L/mol, derived from the
Scatchard plot analysis (26).
Scatchard plot analysis [14] of the purified antiserum exposed a homogeneous antibody population.
The
Scatchard plots in the absence and the presence of sodium chloride are shown in Figures 11 and 12, and the data of n and K are listed in Table 5.
For higher affinity sites, linear equation is qe/Ce=-0.0159qe+0.2323 (R2= 0.9728), and the values of Kd and qmax, calculated from the slope and intercept of
Scatchard plots, are 62.893mg/L and 14.610 mg/g, respectively.
