The data were evaluated in the form of a Scatchard plot, to determine the affinity and binding capacity of the imprinted polymer, expressed by affinity constants (K) and apparent maximum number ([B.
The Scatchard plot of the batch-binding experiments shows that in the imprinted S-MIPs there are two classes of binding sites (visible as two tangents).
Rosenthal transformations of the data are presented as Scatchard plot
for illustration (Figure 1B).
The resulting Scatchard plot
indicated that albumin had 1 high-affinity site and perhaps 2 or more weaker sites (2).
The unchanged structure-activity relations of peptide binding suggest that the detergent did not adversely affect the ligand binding site, but the curvilinear Scatchard plot
does indicate some heterogeneity.
analysis  of the purified antiserum exposed a homogeneous antibody population.
9728), and the values of Kd and qmax, calculated from the slope and intercept of Scatchard plots
, are 62.
But although Scatchard plots
show that cadmium's affinity for the receptor is quite high (dissociation constant is [10.
5 [micro]M were indicated by biphasic Scatchard plots
Figure 4b shows that the Scatchard plots
were only a single straight line, which illustrated that there was only a class of binding sites populated in the nonimprinted microspheres.
were fitted by linear regression analysis and compared using analysis of covariance (ANCOVA).
Individual values of affinity and the coefficient of correlation for linearity of the Scatchard plots
are shown in Table 1.