The data were evaluated in the form of a Scatchard plot, to determine the affinity and binding capacity of the imprinted polymer, expressed by affinity constants (K) and apparent maximum number ([B.
The Scatchard plot of the batch-binding experiments shows that in the imprinted S-MIPs there are two classes of binding sites (visible as two tangents).
Rosenthal transformations of the data are presented as
Scatchard plot for illustration (Figure 1B).
The resulting
Scatchard plot indicated that albumin had 1 high-affinity site and perhaps 2 or more weaker sites (2).
The unchanged structure-activity relations of peptide binding suggest that the detergent did not adversely affect the ligand binding site, but the curvilinear
Scatchard plot does indicate some heterogeneity.
Scatchard plot analysis [14] of the purified antiserum exposed a homogeneous antibody population.
9728), and the values of Kd and qmax, calculated from the slope and intercept of
Scatchard plots, are 62.
But although
Scatchard plots show that cadmium's affinity for the receptor is quite high (dissociation constant is [10.
5 [micro]M were indicated by biphasic
Scatchard plots.
Figure 4b shows that the
Scatchard plots were only a single straight line, which illustrated that there was only a class of binding sites populated in the nonimprinted microspheres.
Scatchard plots were fitted by linear regression analysis and compared using analysis of covariance (ANCOVA).
Individual values of affinity and the coefficient of correlation for linearity of the
Scatchard plots are shown in Table 1.
