MR spectra showed the presence of saccharopine, an intermediate in the Lys degradation pathway activated by the oligomycin treatment in line #163 (Supplementary Figure S3B), in agreement with previous data , and in lines #61 and #74.
A major metabolic difference between the two lines is that line #1, different to line #163, showed almost absent saccharopine formation after oligomycin treatment and belongs to one of the two GSC groups characterized by low/absent adipate content .
Figure S3B and Figure S3C: spectra of saccharopine and lactate signal regions from control and oligomycin-treated #163 line.
Gaillardin, "Cloning of the LYS5 gene encoding saccharopine
dehydrogenase from the yeast Yarrowia lipolytica by target integration," Current Genetics, vol.
Galili, "The catabolic function of the [alpha]-aminoadipic acid pathway in plants is associated with unidirectional activity of lysine-oxoglutarate reductase, but not saccharopine
dehydrogenase," Biochemical Journal, vol.
Although CDSs involved in intermediary metabolism were underrepresented among the deleted genes, 21 (42%) of deleted CDSs of this category were dehydrogenases (such as acyl-CoA short-chain alcohol, saccharopine
, and aldehyde dehydrogenases), which are central enzymes in anaerobic metabolism (22) and important for survival in poorly oxygenated environments such as soil (23).
The major pathway for L-Lys metabolism in the liver occurs through the intermediate saccharopine (Hutzler and Dancis, 1968).
The major pathway of L-Lys metabolism in animals occurs through the intermediate saccharopine. This pathway predominates in the liver and is not very active in the brain (Hutzler and Dancis, 1968).