Preparation of Sabouraud dextrose agar
was done by mixing Sabouraud 4% glucose agar with distilled water by stirring and providing heat.
We instructed participants to take a maximal inspiration and cough twice on each of 2 different agar plates, Sabouraud dextrose agar
and Columbia blood agar, held at a 5-cm distance from the participant's mouth.
All the samples were also subjected to culture on Sabouraud dextrose agar
Recognizing the increase in fungal infections of humans and the decrease of formal training in mycology for clinical laboratory personnel, the bench-side guide and teaching aid describes the macroscopic and microscopic morphologies of cultured fungi as pertaining to those on Sabouraud dextrose agar
. Black and white images depict different types of filamentous bacteria, yeasts, thermally dimorphic fungi, and thermally monomorphic molds.
The segments were then inoculated in Petri plates (90X15 mm) containing different media viz., Czapeck-Dox-Agar (CDA), Potato-Dextrose-Agar (PDA), Malt Extract Agar (MEA), Sabouraud Dextrose Agar
(SDA), V8 juice agar (V8JA), nutrient agar (NA) and water agar media.
* Culture growth from Sabouraud dextrose agar
; see Figure 4, was placed on a Microscan instrument for testing, that later confirmed the organism as C.
Surgical cultures on Sabouraud dextrose agar
grew Sporothrix schenkii within four days confirming the initial diagnosis.
Post-suspension samples were inoculated on to chloramphenicol Sabouraud dextrose agar
(Merck, Germany) and incubated for 48 hours at 37[degrees]C.
One ml from each (prepared sputum, bronchoalveolar lavage, plural fluid) specimens were transferred aseptically into three sterilized nine cm Petri dishes and 15-20 ml of pre-solidifying Sabouraud dextrose agar
medium  were added to each plate.
The Sabouraud Dextrose Agar
(SDA) was prepared in screw cap tubes and autoclaved.
Stock cultures were placed on Sabouraud dextrose agar
(SDA) slant with a 4[degrees]C temperature maintenance.